Human SLX4 Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of SLX4 in RAW264.7 (Mouse) and A431 (Human) cells. SLX4 was detected in immersion fixed RAW 264.7 Mouse Monocyte/Macrophage Cell line (Mouse) and A431 Human Epithelial Carcinoma Cell Line (Human) Cells using Rabbit Anti-Human SLX4 Monoclonal Antibody (Catalog # MAB11246) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
SLX4 (also known as BTBD12 and FANCP) is a multidomain scaffold protein involved in DNA repair, where it has important roles in the final steps of homologous recombination. SLX4 hat interacts with three distinct nucleases: SLX1, ERCC4/XPF-ERCC1 and MUS81-EME1. Mutations in the gene are associated with the disease Fanconi anemia, a rare recessive disorder characterized by genome instability, congenital malformations, progressive bone marrow failure and predisposition to hematologic malignancies and solid tumors.
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