Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 885424
View all Smoothelin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Smoothelin B
Met1-Leu132
Accession # P53814

Specificity

Detects human Smoothelin B in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Smoothelin B Antibody

Smoothelin B antibody in Human Colon by Immunohistochemistry (IHC-P).

Smoothelin B in Human Colon.

Smoothelin B was detected in formalin fixed paraffin-embedded sections of human colon using Mouse Anti-Human Smoothelin B Monoclonal Antibody (Catalog # MAB8278) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle cells in vasculature. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Smoothelin antibody in HASMC Human Cells antibody by Flow Cytometry.

Detection of Smoothelin in HASMC Human Cells by Flow Cytometry.

HASMC human aortic smooth muscle cells was stained with Mouse Anti-Human Smoothelin B Monoclonal Antibody (Catalog # MAB8278, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Intracellular Molecules.

Applications for Human Smoothelin B Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: HASMC human aortic smooth muscle cells

Reviewed Applications

Read 1 review rated 1 using MAB8278 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Smoothelin

Smoothelin (SMTN) is a cytoskeletal protein associated with stress fibers.  It is 917 amino acids (aa) in length and shares 77% and 82% aa identity with mouse and rat SMTN.  SMTN has two major isoforms, A and B with reported molecular weights of approximately 59 and 110 kDa, respectively.  Isoform A is associated with visceral smooth muscle cells while isoform B is specific to vascular smooth muscle.  Loss of SMTN has been associated with defects in intestinal smooth muscle cell contraction as well as increased arterial pressure and cardiac hypertrophy.

Alternate Names

SMSMO, SMTN

Entrez Gene IDs

6525 (Human)

Gene Symbol

SMTN

UniProt

P53814

Additional Smoothelin Products

Product Documents for Human Smoothelin B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Smoothelin B Antibody

For research use only

Customer Reviews for Human Smoothelin B Antibody (1)

1 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
100%

Have you used Human Smoothelin B Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human Smoothelin B Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Aortic smooth muscle cells and HASMC human aortic smooth muscle cells
    Species: Human
    Verified Customer | Posted 03/07/2016
    A wide titration of this Ab was tested on human aortic SMC (Lonza) using an Intercellular Staining Kit (Life Sciences). This Ab was tested negative even though the SMC were stained highly positive for CNN1 (Sigma). Although this Ab did not work, R&D customer service was very helpful and sent a replacement Ab.
    Human Smoothelin B Antibody MAB8278

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies