Human SOX10 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB28642-100
MAB28642-SP
Detection  of SOX10 in U-2 OS Human Cell Line by Flow Cytometry.
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Human SOX10 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SOX10 in direct ELISA.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2447A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human SOX10
Met1-Ala118
Accession # P56693
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection  of SOX10 in U-2 OS Human Cell Line by Flow Cytometry. View Larger

Detection of SOX10 in U-2 OS Human Cell Line by Flow Cytometry. U-2 OS human osteosarcoma cell line was stained with Rabbit Anti-Human SOX10 Monoclonal Antibody (Catalog # MAB28642, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX10

SOX10 belongs to the SOX family of transcription factors which display diverse roles during development. In the central nervous system, SOX10 is required for the terminal differentiation of oligodendrocytes and myelination. In the peripheral nervous system, SOX10 maintains pluripotency of neural crest stem cells and suppresses neuronal differentiation.

References
  1. Lo, L. et al. (2002) Development 129:1553.
Long Name
Transcription Factor SOX10
Entrez Gene IDs
6663 (Human)
Alternate Names
DOM; MGC15649; PCWH; SOX10; SRY (sex determining region Y)-box 10; SRY-related HMG-box gene 10; transcription factor SOX-10; WS4; WS4C; WS4mouse, human homolog of

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