Human SOX3 Antibody
Human SOX3 Antibody Summary
Met1-Leu230
Accession # P41225
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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SOX3 in NTera‑2 Human Cell Line. SOX3 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line differentiation with retinoic acid using Goat Anti-Human SOX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2569) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, upper panel; Catalog # NL003) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOX3
SOX3 belongs to the SOX family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The SOX3 gene is localized on the X chromosome and is expressed in the brain and gonads. It has been suggested that SOX3 plays a role in brain formation and function. Human and mouse SOX3 share 92% amino acid sequence identity.
Product Datasheets
Citations for Human SOX3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Single-cell transcriptomic landscapes of the otic neuronal lineage at multiple early embryonic ages
Authors: Y Sun, L Wang, T Zhu, B Wu, G Wang, Z Luo, C Li, W Wei, Z Liu
Cell Reports, 2022;38(12):110542.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
The Nestin neural enhancer is essential for normal levels of endogenous Nestin in neuroprogenitors but is not required for embryo development
Authors: E Thomson, R Dawson, CH H'ng, F Adikusuma, S Piltz, PQ Thomas
PLoS ONE, 2021;16(11):e0258538.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
An mTORC1-dependent switch orchestrates the transition between mouse spermatogonial stem cells and clones of progenitor spermatogonia
Authors: S Suzuki, JR McCarrey, BP Hermann
Cell Reports, 2021;34(7):108752.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
SOX3 promotes generation of committed spermatogonia in postnatal mouse testes
Authors: D McAninch, JA Mäkelä, HM La, JN Hughes, R Lovell-Bad, RM Hobbs, PQ Thomas
Sci Rep, 2020;10(1):6751.
Species: Mouse
Sample Types: Tissue Homogenate
Applications: Immunoprecipitation -
Identification of dynamic undifferentiated cell states within the male germline
Authors: HM La, JA Mäkelä, AL Chan, FJ Rossello, CM Nefzger, JMD Legrand, M De Seram, JM Polo, RM Hobbs
Nat Commun, 2018;9(1):2819.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Identification of SOX3 as an XX male sex reversal gene in mice and humans.
Authors: Sutton E, Hughes J, White S
J. Clin. Invest., 2011;121(1):328-41.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
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