Human SOX3 Antibody

Catalog # Availability Size / Price Qty
SOX3 in NTera‑2 Human Cell Line.
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Product Details
Citations (6)
Supplemental Products

Human SOX3 Antibody Summary

Species Reactivity
Detects human SOX3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) SOX2, rhSOX7, rhSOX10, and rhSOX17 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human SOX3
Accession # P41225
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human SOX3
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry SOX3 antibody in NTera-2 Human Cell Line by Immunocytochemistry (ICC). View Larger

SOX3 in NTera‑2 Human Cell Line. SOX3 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line differentiation with retinoic acid using Goat Anti-Human SOX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2569) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, upper panel; Catalog # NL003) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX3

SOX3 belongs to the SOX family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The SOX3 gene is localized on the X chromosome and is expressed in the brain and gonads. It has been suggested that SOX3 plays a role in brain formation and function. Human and mouse SOX3 share 92% amino acid sequence identity.

Long Name
Transcription Factor SOX3
Entrez Gene IDs
6658 (Human)
Alternate Names
GHDX; MRGH; panhypopituitarism; PHP; PHPX; SOX3; SOXB; SRY (sex determining region Y)-box 3; transcription factor SOX-3

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Citations for Human SOX3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Single-cell transcriptomic landscapes of the otic neuronal lineage at multiple early embryonic ages
    Authors: Y Sun, L Wang, T Zhu, B Wu, G Wang, Z Luo, C Li, W Wei, Z Liu
    Cell Reports, 2022;38(12):110542.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. The Nestin neural enhancer is essential for normal levels of endogenous Nestin in neuroprogenitors but is not required for embryo development
    Authors: E Thomson, R Dawson, CH H'ng, F Adikusuma, S Piltz, PQ Thomas
    PLoS ONE, 2021;16(11):e0258538.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. An mTORC1-dependent switch orchestrates the transition between mouse spermatogonial stem cells and clones of progenitor spermatogonia
    Authors: S Suzuki, JR McCarrey, BP Hermann
    Cell Reports, 2021;34(7):108752.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. SOX3 promotes generation of committed spermatogonia in postnatal mouse testes
    Authors: D McAninch, JA Mäkelä, HM La, JN Hughes, R Lovell-Bad, RM Hobbs, PQ Thomas
    Sci Rep, 2020;10(1):6751.
    Species: Mouse
    Sample Types: Tissue Homogenate
    Applications: Immunoprecipitation
  5. Identification of dynamic undifferentiated cell states within the male germline
    Authors: HM La, JA Mäkelä, AL Chan, FJ Rossello, CM Nefzger, JMD Legrand, M De Seram, JM Polo, RM Hobbs
    Nat Commun, 2018;9(1):2819.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Identification of SOX3 as an XX male sex reversal gene in mice and humans.
    Authors: Sutton E, Hughes J, White S
    J. Clin. Invest., 2011;121(1):328-41.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr


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