Human Spinesin Antibody

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Product Details
Supplemental Products

Human Spinesin Antibody Summary

Species Reactivity
Detects human Spinesin in direct ELISAs and Western blots.
Monoclonal Mouse IgG1 Clone # 376620
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Spinesin
Tyr27-Leu457 (Phe369Leu)
Accession # Q0P514
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human Spinesin (Catalog # 2495‑SE) under non-reducing conditions only
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Spinesin (Catalog # 2495‑SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Spinesin

Spinesin, encoded by the TMPRSS5 gene, is a member of type II transmembrane serine proteases (TTSPs) (1). Human Spinesin contains the following structural domains: a short N‑terminal cytoplasmic tail (amino acids 1‑49), a transmembrane domain (amino acids 50‑70), a stem region and a serine protease domain (amino acids 71‑457) (2). The domain structure of Spinesin is common to other TTSPs, many of which have additional domains. The stem region of Spinesin contains a scavenger receptor-like domain. The ectodomain of human Spinesin (amino acids 71‑457) was expressed and purified as a single chain pro-enzyme. The deduced amino acid sequence contains a Leu instead of a Phe residue at position 369; the former is identical to the mouse protein (3, 4). The amino acid sequence of human Spinesin is 94%, 82%, 81% and 79% identical to that of chimpanzee, canine, rat and mouse.

  1. Hooper, J.D. et al. (2001) J. Biol. Chem. 276:857.
  2. Yamaguchi, Y. et al. (2002) J. Biol. Chem. 277:6806.
  3. Carninci, P. et al. (2000) Genome Res. 10:1617.
  4. Shibata, K. et al. (2000) Genome Res. 10:1757.
Entrez Gene IDs
80975 (Human); 80893 (Mouse)
Alternate Names
EC 3.4.21; EC 3.4.21.-; EC; MGC141886; MGC148044; Spinesin; TMPRSS5; transmembrane protease serine 5; transmembrane protease, serine 5

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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