Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human STEAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5587) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for STEAP1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human STEAP1 by Simple WesternTM.
Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for STEAP1 at approximately 40 kDa (as indicated) using 10 µg/mL of Goat Anti-Human STEAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5587) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
STEAP1 (six-transmembrane epithelial antigen of the prostate-1) is a 40 kDa protein (predicted) of the STEAP family of metalloreductases. It is expressed mainly at cell‑cell junctions between prostate secretory epithelium, and up‑regulated in prostate and some bladder, colon and ovarian cancers and Ewing’s sarcomas. Human STEAP1 is 339 amino acids (aa) in length. It contains a ferric oxidoreductase domain (aa 119‑265) that includes transmembrane sequences (# 2, 3, 4, and part of 5, out of 6 sequences). Two splice forms diverge at aa 255, terminating after aa 58 or 259. Over aa 1‑71, human and mouse STEAP1 share 68% aa identity.
Six Transmembrane Epithelial Antigen of the Prostate 1
Entrez Gene IDs:
26872 (Human); 70358 (Mouse); 297738 (Rat)
EC 1.16.1.-; PRSS24; PRSS24MGC19484; six transmembrane epithelial antigen of the prostate 1; six transmembrane epithelial antigen of the prostate; Six-transmembrane epithelial antigen of prostate 1; STEAP1; STEAPmetalloreductase STEAP1
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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Western Blot.
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Cancer cell lysates
Other Experimental Details
Other Experimental Details
The antibody worked on Western Blot. A couple of non-specific bands around, but they faint.