Human Supervillin Antibody AF7338: R&D Systems

Human Supervillin Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Supervillin in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Supervillin
    His1801-Thr2214
    Accession # O95425
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Supervillin by Western Blot. Western blot shows lysates of human thyroid tissue and human skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Supervillin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7338) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Supervillin at approximately 240-250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Supervillin

Supervillin ("Largest" villin [family member]; also Archvillin and p250) is a 240-250 kDa member of the villin/gelsolin family of proteins. It is expressed in select cell types, including platelets, neutrophils, renal epithelium and striated muscle cells. Supervillin is found intracellularly in both the nuclear and cytoplasmic compartments. Its principal function is to serve as a scaffold for molecules that interact to oversee changes in the actin cytoskeleton at the cell membrane. It is able to both crosslink F-actin and initiate actin bundle formation. It also binds nonmuscle myosin and negatively regulates focal adhesion complexes. Human supervillin is 2214 amino acids (aa) in length. It contains an NLS (aa 927-931), five gelsolin-like repeats that interact with F-actin (aa 1441-2122), and a C-terminal villin headpiece domain (aa 2179-2214). The 250 kDa form of supervillin (archvillin) is striated muscle-specific. There is also one general nonmuscle form of supervillin (p205) that is 200-210 kDa in size and contains two blocks of deletions between aa 276-669 and 749-781. In ferret, a third 220-230 kDa intermediate isoform has been reported that is unique to smooth muscle. Over aa 1801-2214, human supervillin shares 96% aa sequence identity with mouse supervillin.

  • Entrez Gene IDs:
    6840 (Human); 225115 (Mouse); 361256 (Rat)
  • Alternate Names:
    Archvillin; DKFZp686A17191; membrane-associated F-actin binding protein p205; p205/p250; Supervillin; SVIL
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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