Human SYK (SH2D2 Domain) Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human SYK (SH2D2 Domain) in direct ELISAs. In direct ELISAs, 100% cross-reactivity with the SH2D2 domain of recombinant human (rh) ZAP70 is observed and no cross-reactivity with the SH2 domain of rhCSK is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 720402
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human SYK (SH2D2 Domain)
    Trp168-Cys259
    Accession # P43405
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Simple Western
    20 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse SYK (SH2D2 Domain) by Western Blot. Western blot shows lysates of EL‑4 mouse lymphoblast cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SYK (SH2D2 Domain) Monoclonal Antibody (Catalog # MAB7166) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SYK (SH2D2 Domain) at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Raji human Burkitt's lymphoma cell line 0.2 by Simple WesternTM. Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SYK at approximately 66 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human SYK (SH2D2 Domain) Monoclonal Antibody (Catalog # MAB7166). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse SYK by Simple WesternTM. Simple Western lane view shows lysates of EL‑4 mouse lymphoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for SYK at approximately 66 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human SYK (SH2D2 Domain) Monoclonal Antibody (Catalog # MAB7166). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SYK
SYK (spleen tyrosine kinase) is a 70-80 kDa cytoplasmic non-receptor protein tyrosine kinase of the protein kinase superfamily and the SYK/ZAP-70 subfamily of proteins. The 635 amino acid (aa) human SYK contains two SH2 domains (aa 14-106 and aa 168-259), and one protein kinase domain (aa 371-631). A splicing variant produces a second isoform lacking aa 371-631. Within the second SH2 domain (SH2D2), human SYK shares 94% aa sequence identity with mouse and rat SYK. SYK is widely expressed in hematopoietic cells (notably B lymphocytes), where it couples immunoglobulin receptors to downstream events, such as proliferation, differentiation, and phagocytosis.
  • Long Name:
    Spleen Tyrosine Kinase
  • Entrez Gene IDs:
    6850 (Human); 20963 (Mouse); 25155 (Rat)
  • Alternate Names:
    DKFZp313N1010; EC 2.7.10; EC 2.7.10.2; FLJ25043; spleen tyrosine kinaseFLJ37489; SYK; tyrosine-protein kinase SYK
Related Research Areas
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Mouse IgG Antibody

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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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