Human SYK (SH2D2 Domain) Antibody

Catalog # Availability Size / Price Qty
MAB7166
MAB7166-SP
Human SYK (SH2D2 Domain) Antibody in Data
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Human SYK (SH2D2 Domain) Antibody Summary

Species Reactivity
Human
Specificity
Detects human SYK (SH2D2 Domain) in direct ELISAs. In direct ELISAs, 100% cross-reactivity with the SH2D2 domain of recombinant human (rh) ZAP70 is observed and no cross-reactivity with the SH2 domain of rhCSK is observed.
Source
Monoclonal Mouse IgG1 Clone # 720402
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human SYK (SH2D2 Domain)
Trp168-Cys259
Accession # P43405
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
20 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human and Mouse SYK (SH2D2 Domain) by Western Blot. View Larger

Detection of Human and Mouse SYK (SH2D2 Domain) by Western Blot. Western blot shows lysates of EL-4 mouse lymphoblast cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SYK (SH2D2 Domain) Monoclonal Antibody (Catalog # MAB7166) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SYK (SH2D2 Domain) at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Raji human Burkitt's lymphoma cell line 0.2 by Simple Western<SUP>TM</SUP>. View Larger

Detection of Raji human Burkitt's lymphoma cell line 0.2 by Simple WesternTM. Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SYK at approximately 66 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human SYK (SH2D2 Domain) Monoclonal Antibody (Catalog # MAB7166). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western Detection of Mouse SYK by Simple Western<SUP>TM</SUP>. View Larger

Detection of Mouse SYK by Simple WesternTM. Simple Western lane view shows lysates of EL‑4 mouse lymphoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for SYK at approximately 66 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human SYK (SH2D2 Domain) Monoclonal Antibody (Catalog # MAB7166). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SYK

SYK (spleen tyrosine kinase) is a 70-80 kDa cytoplasmic non-receptor protein tyrosine kinase of the protein kinase superfamily and the SYK/ZAP-70 subfamily of proteins. The 635 amino acid (aa) human SYK contains two SH2 domains (aa 14-106 and aa 168-259), and one protein kinase domain (aa 371-631). A splicing variant produces a second isoform lacking aa 371-631. Within the second SH2 domain (SH2D2), human SYK shares 94% aa sequence identity with mouse and rat SYK. SYK is widely expressed in hematopoietic cells (notably B lymphocytes), where it couples immunoglobulin receptors to downstream events, such as proliferation, differentiation, and phagocytosis.

Long Name
Spleen Tyrosine Kinase
Entrez Gene IDs
6850 (Human); 20963 (Mouse); 25155 (Rat)
Alternate Names
DKFZp313N1010; EC 2.7.10; EC 2.7.10.2; FLJ25043; spleen tyrosine kinaseFLJ37489; SYK; tyrosine-protein kinase SYK

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FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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