Human TAK1 Antibody

Catalog # Availability Size / Price Qty
MAB5307
MAB5307-SP

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Detection of Human TAK1 by Western Blot.
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Product Details
Citations (1)
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Human TAK1 Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human TAK1 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 491840
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TAK1 isoform 1A
Met1-Ser579
Accession # Q5U0D0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TAK1 antibody by Western Blot. View Larger

Detection of Human TAK1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human TAK1 Monoclonal Antibody (Catalog # MAB5307) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TAK1 at approximately 80 to 82 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TAK1

TGF-beta-activated kinase 1 (TAK1; also MAP3K7) is a member of the STE Ser/Thr protein kinase family and the MAP kinase kinase kinase subfamily. Human TAK1 is 606 amino acids (aa) in length and contains a protein kinase domain at aa 36‑291. There are three isoforms for TAK1. Isoform 1B is the largest isoform. Isoform 1A has a 27 aa deletion corresponding to aa 404‑430 in isoform 1B, and isoform 1C has an 88 aa deletion corresponding to aa 519‑606 in isoform 1B. TAK1 activity has been implicated during TGF beta -induced and Ras-dependent tumorigenesis.

Long Name
TGF-beta activated kinase 1
Entrez Gene IDs
6885 (Human); 26409 (Mouse); 313121 (Rat)
Alternate Names
EC 2.7.11; EC 2.7.11.25; MAP3K7; MEKK7; mitogen-activated protein kinase kinase kinase 7; TAK1; TAK1/MAP3K7; TAK1TGF1a; TGF1a; TGF-beta activated kinase 1; TGF-beta-activated kinase 1; Transforming growth factor-beta-activated kinase 1

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Citation for Human TAK1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Phosphorylation and linear ubiquitin direct A20 inhibition of inflammation.
    Authors: Wertz I, Newton K, Seshasayee D, Kusam S, Lam C, Zhang J, Popovych N, Helgason E, Schoeffler A, Jeet S, Ramamoorthi N, Kategaya L, Newman R, Horikawa K, Dugger D, Sandoval W, Mukund S, Zindal A, Martin F, Quan C, Tom J, Fairbrother W, Townsend M, Warming S, DeVoss J, Liu J, Dueber E, Caplazi P, Lee W, Goodnow C, Balazs M, Yu K, Kolumam G, Dixit V
    Nature, 2015-12-09;528(7582):370-5.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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