Human TAK1 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects endogenous human TAK1 in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 491840
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human TAK1 isoform 1A
    Met1-Ser579
    Accession # Q5U0D0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human TAK1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, MCF‑7 human breast cancer cell line, and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human TAK1 Monoclonal Antibody (Catalog # MAB5307) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TAK1 at approximately 80 to 82 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TAK1

TGF-beta-activated kinase 1 (TAK1; also MAP3K7) is a member of the STE Ser/Thr protein kinase family and the MAP kinase kinase kinase subfamily. Human TAK1 is 606 amino acids (aa) in length and contains a protein kinase domain at aa 36‑291. There are three isoforms for TAK1. Isoform 1B is the largest isoform. Isoform 1A has a 27 aa deletion corresponding to aa 404‑430 in isoform 1B, and isoform 1C has an 88 aa deletion corresponding to aa 519‑606 in isoform 1B. TAK1 activity has been implicated during TGF beta -induced and Ras-dependent tumorigenesis.

  • Long Name:
    TGF-beta activated kinase 1
  • Entrez Gene IDs:
    6885 (Human); 26409 (Mouse); 313121 (Rat)
  • Alternate Names:
    EC 2.7.11; EC 2.7.11.25; MAP3K7; MEKK7; mitogen-activated protein kinase kinase kinase 7; TAK1; TAK1/MAP3K7; TAK1TGF1a; TGF1a; TGF-beta activated kinase 1; TGF-beta-activated kinase 1; Transforming growth factor-beta-activated kinase 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Phosphorylation and linear ubiquitin direct A20 inhibition of inflammation.
    Authors: Wertz I, Newton K, Seshasayee D, Kusam S, Lam C, Zhang J, Popovych N, Helgason E, Schoeffler A, Jeet S, Ramamoorthi N, Kategaya L, Newman R, Horikawa K, Dugger D, Sandoval W, Mukund S, Zindal A, Martin F, Quan C, Tom J, Fairbrother W, Townsend M, Warming S, DeVoss J, Liu J, Dueber E, Caplazi P, Lee W, Goodnow C, Balazs M, Yu K, Kolumam G, Dixit V
    Nature, 2015;528(7582):370-5.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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