Human TCF-12/HTF4 Antibody

Catalog # Availability Size / Price Qty
TCF-12/HTF4 in HeLa Human Cell Line.
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Product Details
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Human TCF-12/HTF4 Antibody Summary

Species Reactivity
Detects human TCF-12/HTF4 in direct ELISAs. In direct ELISAs, 75%‑100% cross-reactivity with recombinant human (rh) TCF-3 and no cross-reactivity with rhTCF-1, -2, -4, or rhStathmin-2 is observed.
Monoclonal Mouse IgG1 Clone # 690129
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human TCF-12/HTF4
Accession # Q99081
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry TCF-12/HTF4 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

TCF-12/HTF4 in HeLa Human Cell Line. TCF-12/HTF4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human TCF-12/HTF4 Monoclonal Antibody (Catalog # MAB69281) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TCF-12/HTF4

TCF-12 (transcription factor 12), also called HTF4, bHLHb20 and HEB, is a 682 amino acid (aa) DNA binding protein of the E protein family of helix-loop-helix (HLH) transcription factors. E proteins, including TCF-12 and E2A, mediate lymphocyte differentiation and also function in neurogenesis. Homo-oligomerization is required for activity; hetero-oligomers are also formed. The region of human TCF-12 used as an immunogen excludes the HLH domain and shares 94% aa sequence identity with mouse and rat TCF-12. Potential isoforms of 706 and 648 aa contain an insertion of 24 aa after aa 396, while isoforms of 648, 624 and 512 aa show alternate N‑terminal sequences that merge with the 682 aa form at aa 75, 75 and 194, respectively.

Long Name
Transcription Factor 12
Entrez Gene IDs
6938 (Human); 21406 (Mouse); 25720 (Rat)
Alternate Names
BHLHB20; bHLHb20E-box-binding protein; Class B basic helix-loop-helix protein 20; HEB; HEBDNA-binding protein HTF4; helix-loop-helix transcription factor 4; HsT17266; HTF4; HTF4TCF-12; TCF12; TCF-12; transcription factor 12; Transcription factor HTF-4

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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