Human TFF1 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
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- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Human TFF1 ELISA Standard Curve
Preparation and Storage
The trefoil factor (TFF) family contains three secreted, protease-resistant polypeptides that help to maintain gastrointestinal system integrity. Family members are 7-12 kDa in size, and contain a 3-disulfide bond generated trefoil structure that resembles a meadow clover. TFF1, 2 and 3 are produced in different areas of the digestive tract, and promote restitution, or the resealing of superficial wounds after injury.
Citations for Human TFF1 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Trefoil factor(s) and CA19.9: A promising panel for early detection of pancreatic cancer
Authors: R Jahan, K Ganguly, LM Smith, P Atri, J Carmicheal, Y Sheinin, S Rachagani, G Natarajan, RE Brand, MA Macha, PM Grandgenet, S Kaur, SK Batra
Sample Types: Serum
Trefoil Factor 1 Excretion Is Increased in Early Stages of Chronic Kidney Disease.
Authors: Lebherz-Eichinger D, Tudor B, Ankersmit H, Reiter T, Haas M, Roth-Walter F, Krenn C, Roth G
PLoS ONE, 2015;10(9):e0138312.
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