Human TFF1 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Human TFF1 ELISA Standard Curve
Preparation and Storage
The trefoil factor (TFF) family contains three secreted, protease-resistant polypeptides that help to maintain gastrointestinal system integrity. Family members are 7-12 kDa in size, and contain a 3-disulfide bond generated trefoil structure that resembles a meadow clover. TFF1, 2 and 3 are produced in different areas of the digestive tract, and promote restitution, or the resealing of superficial wounds after injury.
Citations for Human TFF1 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
Filter your results:
Trefoil factor(s) and CA19.9: A promising panel for early detection of pancreatic cancer
Authors: R Jahan, K Ganguly, LM Smith, P Atri, J Carmicheal, Y Sheinin, S Rachagani, G Natarajan, RE Brand, MA Macha, PM Grandgenet, S Kaur, SK Batra
Sample Types: Serum
Trefoil Factor 1 Excretion Is Increased in Early Stages of Chronic Kidney Disease.
Authors: Lebherz-Eichinger D, Tudor B, Ankersmit H, Reiter T, Haas M, Roth-Walter F, Krenn C, Roth G
PLoS ONE, 2015;10(9):e0138312.
No product specific FAQs exist for this product, however you mayView all ELISA FAQs
DuoSet Ancillary Reagent Kits
Supplemental ELISA Products
Reviews for Human TFF1 DuoSet ELISA
There are currently no reviews for this product. Be the first to review Human TFF1 DuoSet ELISA and earn rewards!
Have you used Human TFF1 DuoSet ELISA?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image