Human TFPI-2 Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (2)
Supplemental Products

Human TFPI-2 Antibody Summary

Species Reactivity
Detects human TFPI‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant mouse TFPI-2 is observed and less than 5% cross‑reactivity with recombinant human TFPI‑1 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human TFPI-2
Accession # P48307
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human TFPI‑2 (Catalog # 2545-PI)
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human TFPI‑2 (Catalog # 2545‑PI), see our available Western blot detection antibodies
Measured by its ability to neutralize Recombinant Human TFPI-2 (0.17 µg/mL, Catalog # 2545-PI) inhibition of Trypsin (0.05 µg/mL) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002 ). The Neutralization Dose (ND50) is typically 1.4 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFPI-2

Human Tissue Factor Pathway Inhibitor 2 (TFPI-2), also known as placental protein 5 (PP5) and retinal pigment epithelial cell factor 1 (REF-1), is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains (residues 36 to 86, 96 to 149 and 158 to 208) separated with by two linker regions, and a C-terminal basic region (1‑3). Expression of TFPI-2 is down-regulated in several cancers, which may contribute to tumor progression in these cancers (4). The purified recombinant human TFPI-2 ends at residue 213 and does not contain the last 22 residues (residues 214 to 235) in the C-terminal region. It inhibits the activity of recombinant human Coagulation Factor VII (R&D Systems, Catalog # 2338-SE) in the presence of recombinant human Tissue Factor (R&D Systems, Catalog # 2339-PA).

  1. Miyagi, Y. et al. (1994) J. Biochem. 116:939.
  2. Sprecher, C.A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:3353.
  3. Tanaka, Y. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45:245.
  4. Rollin, J. et al. (2005) Br, J. Cancer 92:775.
Long Name
Tissue Factor Pathway Inhibitor 2
Entrez Gene IDs
7980 (Human)
Alternate Names
Placental protein 5; PP5; PP5FLJ21164; REF-1; TFPI2; TFPI-2; TFPI-2REF1; tissue factor pathway inhibitor 2

Product Datasheets

Citations for Human TFPI-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. TFPI-2 suppresses breast cancer cell proliferation and invasion through regulation of ERK signaling and interaction with actinin-4 and myosin-9
    Authors: G Wang, W Huang, W Li, S Chen, W Chen, Y Zhou, P Peng, W Gu
    Sci Rep, 2018;8(1):14402.
    Species: Human
    Sample Types: Cell Lysates
  2. Use of an immunoaffinity-mass spectrometry-based approach for the quantification of protein biomarkers from serum samples of lung cancer patients.
    Authors: Nicol GR, Han M, Kim J, Birse CE, Brand E, Nguyen A, Mesri M, FitzHugh W, Kaminker P, Moore PA, Ruben SM, He T
    Mol. Cell Proteomics, 2008;7(10):1974-82.
    Species: Human
    Sample Types: Serum
    Applications: Mass Spectrometry


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