Human TFPI-2 Antibody

R&D Systems | Catalog # AF2545

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Neutralization, Immunoprecipitation

Cited:

Mass Spectrometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TFPI-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TFPI-2
Asp23-Lys213
Accession # P48307

Specificity

Detects human TFPI‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant mouse TFPI-2 is observed and less than 5% cross‑reactivity with recombinant human TFPI‑1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications for Human TFPI-2 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human TFPI‑2 (Catalog # 2545‑PI), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human TFPI‑2 (Catalog # 2545-PI)

Neutralization

Measured by its ability to neutralize Recombinant Human TFPI-2 (0.17 µg/mL, Catalog # 2545-PI) inhibition of Trypsin (0.05 µg/mL) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002 ). The Neutralization Dose (ND50) is typically 1.4 µg/mL.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TFPI-2

Human Tissue Factor Pathway Inhibitor 2 (TFPI-2), also known as placental protein 5 (PP5) and retinal pigment epithelial cell factor 1 (REF-1), is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains (residues 36 to 86, 96 to 149 and 158 to 208) separated with by two linker regions, and a C-terminal basic region (1‑3). Expression of TFPI-2 is down-regulated in several cancers, which may contribute to tumor progression in these cancers (4). The purified recombinant human TFPI-2 ends at residue 213 and does not contain the last 22 residues (residues 214 to 235) in the C-terminal region. It inhibits the activity of recombinant human Coagulation Factor VII (R&D Systems, Catalog # 2338-SE) in the presence of recombinant human Tissue Factor (R&D Systems, Catalog # 2339-PA).

References

  1. Miyagi, Y. et al. (1994) J. Biochem. 116:939.
  2. Sprecher, C.A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:3353.
  3. Tanaka, Y. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45:245.
  4. Rollin, J. et al. (2005) Br, J. Cancer 92:775.

Long Name

Tissue Factor Pathway Inhibitor 2

Alternate Names

PP5, REF-1, TFPI2

Entrez Gene IDs

7980 (Human)

Gene Symbol

TFPI2

UniProt

P48307

Additional TFPI-2 Products

Product Documents for Human TFPI-2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TFPI-2 Antibody

For research use only

Citations for Human TFPI-2 Antibody

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FAQs

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Associated Pathways

Blood Coagulation Signaling Pathways
Blood Coagulation Signaling Pathway Thumbnail