Human TIM-1/KIM-1/HAVCR Antibody

Clone 219211 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB1750-SP
MAB1750-100
MAB1750-500
Human TIM-1/KIM-1/HAVCR Antibody in Western Blot
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Product Details
Citations (15)
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Human TIM-1/KIM-1/HAVCR Antibody Summary

Species Reactivity
Human
Specificity
Detects human TIM‑1/KIM-1/HAVCR in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) TIM-1, rmTIM-2, or rhTIM-3.
Source
Monoclonal Mouse IgG2B Clone # 219211
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIM‑1/KIM-1/HAVCR
Ser21-Thr288
Accession # AAC39862
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human TIM‑1/KIM‑1/HAVCR by Western Blot. View Larger

Detection of Human TIM‑1/KIM‑1/HAVCR by Western Blot. Western blot shows lysates of human CD4+cells treated (+) with 5 µg/mL of Hamster Anti-Mouse CD3e Monoclonal Antibody (Catalog # MAB484) and 1 µg/mL of Rat Anti-Mouse CD28 Monoclonal Antibody (Catalog # MAB4831) for 24 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TIM-1/KIM-1/HAVCR Monoclonal Antibody (Catalog # MAB1750) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TIM-1/KIM-1/HAVCR at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of TIM‑1/KIM‑1/HAVCR in Th2-stimulated Human PBMCs by Flow Cytometry. View Larger

Detection of TIM‑1/KIM‑1/HAVCR in Th2-stimulated Human PBMCs by Flow Cytometry. (A) Unstimulated and (B) Th2-stimulated human PBMCs were stained with Mouse Anti-Human TIM-1/KIM-1/HAVCR Monoclonal Antibody (Catalog # MAB1750) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Human CD4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB3791C). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).

Immunohistochemistry TIM‑1/KIM‑1/HAVCR in Human Kidney. View Larger

TIM‑1/KIM‑1/HAVCR in Human Kidney. TIM-1/KIM-1/HAVCR was detected in immersion fixed paraffin-embedded sections of human kidney using 25 µg/mL Mouse Anti-Human TIM-1/ KIM-1/HAVCR Monoclonal Antibody (Catalog # MAB1750) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-1/KIM-1/HAVCR

TIM-1 (T cell-immunoglobulin-mucin; also KIM-1 and HAVcr-1) is a 100 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-3). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. Human TIM-1 is synthesized as a 359 amino acid (aa) precursor that contains a 20 aa signal sequence, a 270 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 48 aa cytoplasmic domain (4-6). The ECD contains oneV-type Ig-like domain and a mucin region characterized by multiple PTTTTL motifs. The mucin region undergoes extensive O-linked glycosylation. The TIM-1 gene is highly polymorphic and undergoes alternate splicing (1). For instance, the presence of a six aa sequence (MTTTVP) at position #137 of the mature molecule is associated with protection from atopy in people with a history of hepatitis A (7, 8). There are two cytoplasmic alternate splice forms of
TIM‑1. One is a long (359 aa) kidney form termed TIM-1b, and one is a short (334 aa) liver form termed TIM-1a. Both are identical through the first 323 aa of their precursors. TIM-1b contains a tyrosine phosphorylation motif that is not present in 1a (6). TIM-1 is also known to circulate as a soluble form. Constitutive cleavage by an undefined MMP (possibly ADAM33) releases an 85 - 90 kDa soluble molecule (6). The ECD of human TIM-1 is 50% and 43% aa identical to mouse and canine TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (4, 9). However, others are believed to exist, and based on the ligand for TIM-3, one may well be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10).

References
  1. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471.
  2. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454.
  3. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
  4. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
  5. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
  6. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
  7. Umetsu, D.T. et al. (2005) J. Pediatr. Gastroenterol. Nutr. 40:S43.
  8. Gao, P-S. et al. (2005) J. Allergy Clin. Immunol. 115:982.
  9. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  10. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
Long Name
T Cell Immunoglobulin Mucin-1
Entrez Gene IDs
26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)
Alternate Names
CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1

Product Datasheets

Citations for Human TIM-1/KIM-1/HAVCR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Dynamic Dystroglycan Complexes Mediate Cell Entry of Lassa Virus
    Authors: A Herrador, C Fedeli, E Radulovic, KP Campbell, H Moreno, G Gerold, S Kunz
    MBio, 2019;10(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  2. TIM-1 Promotes Japanese Encephalitis Virus Entry and Infection
    Authors: J Niu, Y Jiang, H Xu, C Zhao, G Zhou, P Chen, R Cao
    Viruses, 2018;10(11):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy
    Authors: O Maydan, PG McDade, Y Liu, XR Wu, DG Matsell, AA Eddy
    Physiol Rep, 2018;6(6):e13654.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Attachment and Post-Attachment Receptors Important for Hepatitis C Virus Infection and Cell-to-Cell Transmission
    Authors: H Fan, L Qiao, KD Kang, J Fan, W Wei, G Luo
    J. Virol., 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. TIM1 (HAVCR1) Is Not Essential for Cellular Entry of Either Quasi-enveloped or Naked Hepatitis A Virions
    Authors: A Das, A Hirai-Yuki, O González-L, B Rhein, S Moller-Tan, R Brouillett, L Hensley, I Misumi, W Lovell, JM Cullen, JK Whitmire, W Maury, SM Lemon
    MBio, 2017;8(5):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication
    Authors: KM Aagaard, A Lahon, MA Suter, RP Arya, MD Seferovic, MB Vogt, M Hu, F Stossi, MA Mancini, RA Harris, M Kahr, C Eppes, M Rac, MA Belfort, CS Park, D Lacorazza, R Rico-Hesse
    Sci Rep, 2017;7(0):41389.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Ebola Virus Binding to Tim-1 on T Lymphocytes Induces a Cytokine Storm.
    Authors: Patrick Younan, Mathieu Iampietro, Andrew Nishida, Palaniappan Ramanatha, Rodrigo I Santos, Mukta Dutta, Ndongala Michel Lubaki, Richard A Koup, Michael G Katze, Alexander Bukreyev
    MBio, 2017;0(0):2150-7511.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. TIM-1 promotes hepatitis C virus cell attachment and infection
    Authors: Guangxiang Luo
    J. Virol., 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Expression of Tim-1 in primary CNS lymphoma
    Cancer Med, 2016;5(11):3235-3245.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Laser capture microdissection and multiplex-tandem PCR analysis of proximal tubular epithelial cell signaling in human kidney disease.
    Authors: Wilkinson R, Wang X, Kassianos A, Zuryn S, Roper K, Osborne A, Sampangi S, Francis L, Raghunath V, Healy H
    PLoS ONE, 2014;9(1):e87345.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Acute pretreatment with chloroquine attenuates renal I/R injury in rats.
    Authors: Todorovic, Zoran, Medic, Branisla, Basta-Jovanovic, Gordana, Radojevic Skodric, Sanja, Stojanovic, Radan, Rovcanin, Branisla, Prostran, Milica
    PLoS ONE, 2014;9(3):e92673.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. A polymorphism of the TIM-1 IgV domain: implications for the susceptibility to filovirus infection.
    Authors: Kuroda M, Fujikura D, Noyori O, Kajihara M, Maruyama J, Miyamoto H, Yoshida R, Takada A
    Biochem Biophys Res Commun, 2014;455(3):223-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. TIM-family proteins promote infection of multiple enveloped viruses through virion-associated phosphatidylserine.
    Authors: Jemielity, Stephani, Wang, Jinyize, Chan, Ying Kai, Ahmed, Asim A, Li, Wenhui, Monahan, Sheena, Bu, Xia, Farzan, Michael, Freeman, Gordon J, Umetsu, Dale T, Dekruyff, Rosemari, Choe, Hyeryun
    PLoS Pathog, 2013;9(3):e1003232.
  14. Gene-trap mutagenesis identifies mammalian genes contributing to intoxication by Clostridium perfringens epsilon-toxin.
    Authors: Ivie SE, Fennessey CM, Sheng J, Rubin DH, McClain MS
    PLoS ONE, 2011;6(3):e17787.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell-based ELISA
  15. Altered expression of T cell immunoglobulin-mucin (TIM) molecules in bronchoalveolar lavage CD4+ T cells in sarcoidosis.
    Authors: Idali F, Wahlstrom J, Dahlberg B, Khademi M, Olsson T, Eklund A, Grunewald J
    Respir Res, 2009;10(0):42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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