Detection of Human TIM‑1/KIM‑1/HAVCR by Western Blot. Western blot shows lysates of human CD4+ cells treated (+) with 5 µg/mL of Hamster Anti-Mouse CD3 epsilon Monoclonal Antibody (Catalog # MAB484) and 1 µg/mL of Rat Anti-Mouse CD28 Monoclonal Antibody (Catalog # MAB4831) for 24 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TIM‑1/KIM‑1/HAVCR Monoclonal Antibody (Catalog # MAB1750) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TIM‑1/KIM‑1/HAVCR at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of TIM‑1/KIM‑1/HAVCR in Th2-stimulated Human PBMCs by Flow Cytometry. (A) Unstimulated and (B) Th2-stimulated human PBMCs were stained with Mouse Anti-Human TIM‑1/KIM‑1/HAVCR Monoclonal Antibody (Catalog # MAB1750) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Human CD4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB3791C). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).
TIM‑1/KIM‑1/HAVCR in Human Kidney. TIM‑1/KIM‑1/HAVCR was detected in immersion fixed paraffin-embedded sections of human kidney using 25 µg/mL Mouse Anti-Human TIM‑1/ KIM‑1/HAVCR Monoclonal Antibody (Catalog # MAB1750) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TIM-1 (T cell-immunoglobulin-mucin; also KIM-1 and HAVcr-1) is a 100 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-3). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. Human TIM-1 is synthesized as a 359 amino acid (aa) precursor that contains a 20 aa signal sequence, a 270 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 48 aa cytoplasmic domain (4-6). The ECD contains oneV-type Ig-like domain and a mucin region characterized by multiple PTTTTL motifs. The mucin region undergoes extensive O-linked glycosylation. The TIM-1 gene is highly polymorphic and undergoes alternate splicing (1). For instance, the presence of a six aa sequence (MTTTVP) at position #137 of the mature molecule is associated with protection from atopy in people with a history of hepatitis A (7, 8). There are two cytoplasmic alternate splice forms of TIM‑1. One is a long (359 aa) kidney form termed TIM-1b, and one is a short (334 aa) liver form termed TIM-1a. Both are identical through the first 323 aa of their precursors. TIM-1b contains a tyrosine phosphorylation motif that is not present in 1a (6). TIM-1 is also known to circulate as a soluble form. Constitutive cleavage by an undefined MMP (possibly ADAM33) releases an 85 - 90 kDa soluble molecule (6). The ECD of human TIM-1 is 50% and 43% aa identical to mouse and canine TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (4, 9). However, others are believed to exist, and based on the ligand for TIM-3, one may well be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10).
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