Human TIM-1/KIM-1/HAVCR Antibody MAB1750-100: R&D Systems

Human TIM-1/KIM-1/HAVCR Antibody

Clone 219211 was used by HLDA to establish CD designation.
(8 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human TIM‑1/KIM-1/HAVCR in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) TIM-1, rmTIM-2, or rhTIM-3.
  • Source
    Monoclonal Mouse IgG2B Clone # 219211
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TIM‑1/KIM-1/HAVCR
    Ser21-Thr288
    Accession # AAC39862
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human TIM‑1/KIM‑1/HAVCR by Western Blot. Western blot shows lysates of human CD4+ cells treated (+) with 5 µg/mL of Hamster Anti-Mouse CD3 epsilon Monoclonal Antibody (Catalog # MAB484) and 1 µg/mL of Rat Anti-Mouse CD28 Monoclonal Antibody (Catalog # MAB4831) for 24 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TIM‑1/KIM‑1/HAVCR Monoclonal Antibody (Catalog # MAB1750) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TIM‑1/KIM‑1/HAVCR at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of TIM‑1/KIM‑1/HAVCR in Th2-stimulated Human PBMCs by Flow Cytometry. (A) Unstimulated and (B) Th2-stimulated human PBMCs were stained with Mouse Anti-Human TIM‑1/KIM‑1/HAVCR Monoclonal Antibody (Catalog # MAB1750) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Human CD4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB3791C). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).
Immunohistochemistry
TIM‑1/KIM‑1/HAVCR in Human Kidney. TIM‑1/KIM‑1/HAVCR was detected in immersion fixed paraffin-embedded sections of human kidney using 25 µg/mL Mouse Anti-Human TIM‑1/
KIM‑1/HAVCR Monoclonal Antibody (Catalog # MAB1750) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIM-1/KIM-1/HAVCR

TIM-1 (T cell-immunoglobulin-mucin; also KIM-1 and HAVcr-1) is a 100 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-3). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. Human TIM-1 is synthesized as a 359 amino acid (aa) precursor that contains a 20 aa signal sequence, a 270 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 48 aa cytoplasmic domain (4-6). The ECD contains oneV-type Ig-like domain and a mucin region characterized by multiple PTTTTL motifs. The mucin region undergoes extensive O-linked glycosylation. The TIM-1 gene is highly polymorphic and undergoes alternate splicing (1). For instance, the presence of a six aa sequence (MTTTVP) at position #137 of the mature molecule is associated with protection from atopy in people with a history of hepatitis A (7, 8). There are two cytoplasmic alternate splice forms of
TIM‑1. One is a long (359 aa) kidney form termed TIM-1b, and one is a short (334 aa) liver form termed TIM-1a. Both are identical through the first 323 aa of their precursors. TIM-1b contains a tyrosine phosphorylation motif that is not present in 1a (6). TIM-1 is also known to circulate as a soluble form. Constitutive cleavage by an undefined MMP (possibly ADAM33) releases an 85 - 90 kDa soluble molecule (6). The ECD of human TIM-1 is 50% and 43% aa identical to mouse and canine TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (4, 9). However, others are believed to exist, and based on the ligand for TIM-3, one may well be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10).

  • References:
    1. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471.
    2. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454.
    3. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
    4. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
    5. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
    6. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
    7. Umetsu, D.T. et al. (2005) J. Pediatr. Gastroenterol. Nutr. 40:S43.
    8. Gao, P-S. et al. (2005) J. Allergy Clin. Immunol. 115:982.
    9. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
    10. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
  • Long Name:
    T Cell Immunoglobulin Mucin-1
  • Entrez Gene IDs:
    26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)
  • Alternate Names:
    CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication
    Authors: KM Aagaard, A Lahon, MA Suter, RP Arya, MD Seferovic, MB Vogt, M Hu, F Stossi, MA Mancini, RA Harris, M Kahr, C Eppes, M Rac, MA Belfort, CS Park, D Lacorazza, R Rico-Hesse
    Sci Rep, 2017;7(0):41389.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. Expression of Tim-1 in primary CNS lymphoma
    Cancer Med, 2016;5(11):3235-3245.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  3. Acute pretreatment with chloroquine attenuates renal I/R injury in rats.
    Authors: Todorovic, Zoran, Medic, Branisla, Basta-Jovanovic, Gordana, Radojevic Skodric, Sanja, Stojanovic, Radan, Rovcanin, Branisla, Prostran, Milica
    PLoS ONE, 2014;9(3):e92673.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. Laser capture microdissection and multiplex-tandem PCR analysis of proximal tubular epithelial cell signaling in human kidney disease.
    Authors: Wilkinson R, Wang X, Kassianos A, Zuryn S, Roper K, Osborne A, Sampangi S, Francis L, Raghunath V, Healy H
    PLoS ONE, 2014;9(1):e87345.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  5. A polymorphism of the TIM-1 IgV domain: implications for the susceptibility to filovirus infection.
    Authors: Kuroda M, Fujikura D, Noyori O, Kajihara M, Maruyama J, Miyamoto H, Yoshida R, Takada A
    Biochem Biophys Res Commun, 2014;455(3):223-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. TIM-family proteins promote infection of multiple enveloped viruses through virion-associated phosphatidylserine.
    Authors: Jemielity, Stephani, Wang, Jinyize, Chan, Ying Kai, Ahmed, Asim A, Li, Wenhui, Monahan, Sheena, Bu, Xia, Farzan, Michael, Freeman, Gordon J, Umetsu, Dale T, Dekruyff, Rosemari, Choe, Hyeryun
    PLoS Pathog, 2013;9(3):e1003232.
  7. Gene-trap mutagenesis identifies mammalian genes contributing to intoxication by Clostridium perfringens epsilon-toxin.
    Authors: Ivie SE, Fennessey CM, Sheng J, Rubin DH, McClain MS
    PLoS ONE, 2011;6(3):e17787.
    Species: Human
    Sample Type: Whole Cells
    Application: Cell-based ELISA
  8. Altered expression of T cell immunoglobulin-mucin (TIM) molecules in bronchoalveolar lavage CD4+ T cells in sarcoidosis.
    Authors: Idali F, Wahlstrom J, Dahlberg B, Khademi M, Olsson T, Eklund A, Grunewald J
    Respir Res, 2009;10(0):42.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
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