Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 812417
View all TINAGL1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human TINAGL1
Ala22-Met464
Accession # Q9GZM7

Specificity

Detects human TINAGL1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human TIN-Ag is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TINAGL1 Antibody

Detection of Human TINAGL1 antibody by Western Blot.

Detection of Human TINAGL1 by Western Blot.

Western blot shows lysates of human placenta tissue and human kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human TINAGL1 Monoclonal Antibody (Catalog # MAB7185) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TINAGL1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human TINAGL1 antibody by Simple WesternTM.

Detection of Human TINAGL1 by Simple WesternTM.

Simple Western lane view shows lysates of human placenta tissue, loaded at 0.5 mg/mL. A specific band was detected for TINAGL1 at approximately 59 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human TINAGL1 Monoclonal Antibody (Catalog # MAB7185). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Human TINAGL1 Antibody

Application
Recommended Usage

Simple Western

5 µg/mL
Sample: Human placenta tissue

Western Blot

0.5 µg/mL
Sample: Human placenta tissue and human kidney tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TINAGL1

TINAGL1 (Tubulointerstitial nephritis antigen-like 1; also TIN-ag-RP, AZ-1 and LCN7) is a 54-57 kDa glycoprotein member of the peptidase C1 family of molecules. It is secreted by renal tubule epithelium, zona glomerulosa cells, vascular smooth muscle and striated muscle cells. TINAGL1 may play a role in the spatial and temporal development of the steroid component of the adrenal, in part by suppressing the expression of CYP11B1. Human TINAGL1 is synthesized as a 467 amino acid (aa) precursor. It contains a 21 aa signal sequence and a 446 aa mature region (aa 22-467). Within the mature region is an SMB (somatomedin B) domain (aa 50-98), one vWFC domain (aa 105-140), and a nonenzymatic peptidase C1A region (aa 204-455). There are multiple potential isoform variants. One contains an alternative start site at Met106, another shows a deletion of aa 126-156, and a third possesses a 60 aa substitution for aa 1-194, coupled to a 38 aa insertion after Gly286, and a 16 aa substitution for aa 365-467. Undefined forms of 40-42 kDa and 22 kDa  have been noted in TINAGL1-transfected cell supernatant subjected to SDS-PAGE. Over aa 22‑464, human TINAGL1 shares 90% aa identity with mouse TINAGL1.

Long Name

Tubulointerstitial Nephritis Antigen-like 1

Alternate Names

GIS5, LCN7, LIECG3, OLRG2, P3ECSL, TIN-ag-RP

Entrez Gene IDs

64129 (Human); 94242 (Mouse); 94174 (Rat)

Gene Symbol

TINAGL1

UniProt

Q9GZM7 (Human)

Additional TINAGL1 Products

Product Documents for Human TINAGL1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TINAGL1 Antibody

For research use only

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