Human Total ErbB4/Her4 DuoSet IC ELISA

Catalog # Availability Size / Price Qty
DYC1133-5
DYC1133-2
Product Details
Citations (1)
FAQs
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Human Total ErbB4/Her4 DuoSet IC ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4 hours 40 mins (after plate preparation)
Sample Volume Required
Cell lysates (100 µL)
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total ErbB4 / Her4 in cell lysates. An immobilized capture antibody specific for ErbB4 / Her4 binds both phosphorylated and unphosphorylated ErbB4 / Her4. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot

Kit Content

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*


Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

 

Data Examples
DYC1133 DuoSet IC ELISA Figure 1
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Figure 1: The Human Total ErbB4 DuoSet IC ELISA is more sensitive than Western blot analysis. Lysates prepared from MDA-MB-453 human breast cancer cells were serially diluted and analyzed by (A) Western Blot and (B) this DuoSet® IC ELISA. Immunoblots were incubated with an anti-ErbB4 polyclonal antibody to detect total ErbB4. Bands were visualized with anti-rabbit IgG-HRP (Catalog # HAF008) followed by chemiluminescent detection. Human ErbB4 can be detected in this DuoSet® IC ELISA by using approximately 5 to 10 times less lysate than is needed for a conventional IP-Western Blot.

DYC1133 DuoSet IC ELISA Figure 2
View Larger Image

Figure 2: The Human Total ErbB4 DuoSet IC ELISA measures the relative level of ErbB4. Lysates were prepared from MDA-MB-453 human breast cancer cells and the MCF-7 human breast cancer cell line. Both ELISA and Western Blot (inset) analyses were performed using 100 g of lysate. The Western Blot was performed as described in Figure 1.

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ErbB4/Her4

ErbB4, also known as Her4, is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the EGF family of growth factors. ErbB4 ligands include the neuregulins, betacellulin and heparin-binding EGF-like growth factor (HB-EGF).

Long Name:
Receptor Tyrosine Protein Kinase ErbB4
Entrez Gene IDs:
2066 (Human); 13869 (Mouse); 59323 (Rat)
Alternate Names:
avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; EC 2.7.10; EC 2.7.10.1; ErbB4; HER4; HER4MGC138404; p180erbB4; Proto-oncogene-like protein c-ErbB-4; receptor tyrosine-protein kinase erbB-4; Tyrosine kinase-type cell surface receptor HER4; v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 4; v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)

Citation for Human Total ErbB4/Her4 DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Assessment of a combination of Serum Proteins as potential biomarkers to clinically predict Schizophrenia
    Authors: C Li, H Tao, X Yang, X Zhang, Y Liu, Y Tang, A Tang
    Int J Med Sci, 2018;15(9):900-906.
    Species: Human
    Sample Types: Serum

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