Human Total ErbB4/Her4 DuoSet IC ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Available in 2, 5, and 15- (96-well) plate pack sizes
- Economical alternative to Western blot
- Capture Antibody
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard or Control
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product
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Figure 1: The Human Total ErbB4 DuoSet IC ELISA is more sensitive than Western blot analysis. Lysates prepared from MDA-MB-453 human breast cancer cells were serially diluted and analyzed by (A) Western Blot and (B) this DuoSet® IC ELISA. Immunoblots were incubated with an anti-ErbB4 polyclonal antibody to detect total ErbB4. Bands were visualized with anti-rabbit IgG-HRP (Catalog # HAF008) followed by chemiluminescent detection. Human ErbB4 can be detected in this DuoSet® IC ELISA by using approximately 5 to 10 times less lysate than is needed for a conventional IP-Western Blot.
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Figure 2: The Human Total ErbB4 DuoSet IC ELISA measures the relative level of ErbB4. Lysates were prepared from MDA-MB-453 human breast cancer cells and the MCF-7 human breast cancer cell line. Both ELISA and Western Blot (inset) analyses were performed using 100 g of lysate. The Western Blot was performed as described in Figure 1.
Preparation and Storage
ErbB4, also known as Her4, is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the EGF family of growth factors. ErbB4 ligands include the neuregulins, betacellulin and heparin-binding EGF-like growth factor (HB-EGF).
Citation for Human Total ErbB4/Her4 DuoSet IC ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Assessment of a combination of Serum Proteins as potential biomarkers to clinically predict Schizophrenia
Authors: C Li, H Tao, X Yang, X Zhang, Y Liu, Y Tang, A Tang
Int J Med Sci, 2018;15(9):900-906.
Sample Types: Serum
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