Human Total Progesterone R/NR3C3 DuoSet IC ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
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- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Available in 2, 5, and 15- (96-well) plate pack sizes
- Economical alternative to Western blot
- Capture Antibody
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard or Control
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Preparation and Storage
Background: Progesterone R/NR3C3
The progesterone Receptor (PR; NR3C3) is a member of the steroid receptor superfamily which binds progesterone. The progesterone receptor is expressed in uterus, ovary, vagina, fallopian tubes and breast. Progesterone receptors play a central role in diverse reproductive events associated with the establishment and maintenance of pregnancy, alveolar development in the breast, and sexual behavior. Two isoforms of the progesterone receptor have been identified, progesterone receptor-A (PR-A) and progesterone receptor-B (PR-B), 94 and 114 kDa in size, respectively.
The B progesterone receptor (PR-B), or long form, is 933 amino acids (aa) in length. Progesterone receptor-A (PR-A) utilizes a different start site that shortens the N-terminus by 164 amino acids. The N-terminus in both progesterone receptor isoforms is rich in serine that is phosphorylated in response to hormone binding. In the absence of progesterone, few receptor molecules are phosphorylated at Serine 190 (S190). Progesterone increases this number two-fold, providing evidence for hormone stimulation. It has been shown that the two progesterone receptor isoforms can form homodimers as well as heterodimers.
Citation for Human Total Progesterone R/NR3C3 DuoSet IC ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Disposable Amperometric Immunosensor for the Determination of Human P53 Protein in Cell Lysates Using Magnetic Micro-Carriers
Authors: José M Pingarrón
Biosensors (Basel), 2016;6(4):.
Sample Types: Cell Lysates
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