Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TPT1/TCTP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TPT1/TCTP
Met1-Cys172
Accession # P13693

Specificity

Detects human TPT1/TCTP in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse TPT1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TPT1/TCTP Antibody

Detection of Human TPT1/TCTP antibody by Western Blot.

Detection of Human TPT1/TCTP by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TPT1/TCTP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4648) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TPT1/TCTP at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human TPT1/TCTP Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and Nalm‑6 human Pre-B acute lymphocytic leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TPT1/TCTP

TPT-1 (translationally controlled tumor protein 1; also TCTP, HRF and fortilin) is a 22-23 kDa member of the TCTP family of calcium-binding proteins. It is widely expressed (but not in kidney), and apparently serves as a transcriptional activator (Oct4), calcium transporter, histamine inducer and antiapoptotic, caspase-3 inhibitor. Human TPT-1 is 172 amino acids (aa) in length. It contains little in the way of structural motifs. There are multiple potential splice variants. There is an alternate start site at Met35 that may be accompanied by a deletion of aa 99-172. In addition, there may be either a 25 aa or 16 aa C-terminal extension after Cys172. Over aa 1-172, human TPT-1 is 96% aa identical to mouse TPT-1.

Long Name

Translationally-controlled Tumor Protein 1

Alternate Names

Fortilin, HRF, p02, TCTP

Entrez Gene IDs

7178 (Human); 22070 (Mouse); 116646 (Rat)

Gene Symbol

TPT1

UniProt

P13693

Additional TPT1/TCTP Products

Product Documents for Human TPT1/TCTP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TPT1/TCTP Antibody

For research use only

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