Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Neutralization

Cited:

Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 175707
View all TRANCE/TNFSF11/RANK L Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TRANCE/TNFSF11/RANK L
Gly64-Asp245
Accession # AAC51762

Specificity

Detects human TRANCE/TNFSF11/RANK L in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human TRANCE/TNFSF11/RANK L Antibody (175707)

Osteoclast-Like Cell Formation Induced by TRANCE/TNFSF11/RANK L and Neutralization by Human TRANCE/TNFSF11/RANK L Antibody.

Osteoclast-Like Cell Formation Induced by TRANCE/TNFSF11/RANK L and Neutralization by Human TRANCE/TNFSF11/RANK L Antibody.

In the presence of Recombinant Mouse M-CSF (20 ng/mL, Catalog # 416-ML), Recombinant Human TRANCE/TNFSF11/RANK L (Catalog # 390-TN) induces osteoclast-like cell formation in the RAW 264.7 mouse monocyte/macrophage cell line in a dose-dependent manner (orange line), as measured by TRAP (tartrate-resistant acid phosphatase) activity in cell lysates. Under these conditions, osteoclast-like cell formation elicited by Recombinant Human TRANCE/TNFSF11/RANK L (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TRANCE/ TNFSF11/RANK L Monoclonal Antibody (Catalog # MAB6262). The ND50 is typically 0.01-0.04 µg/mL.

Applications for Human TRANCE/TNFSF11/RANK L Antibody (175707)

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize TRANCE/TNFSF11/RANK L-induced osteoclast-like cell formation in the RAW 264.7 mouse monocyte/macrophage cell line. Nakagawa, N. et al. (1998) Biochim. Biophys. Res. Commun. 253:395. The Neutralization Dose (ND50) is typically 0.01-0.04 µg/mL in the presence of 30 ng/mL Recombinant Human TRANCE/TNFSF11/RANK L.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRANCE/TNFSF11/RANK L

TRANCE, also known as RANK ligand, is a type II membrane protein belonging to the tumor necrosis factor superfamily and has been designated TNFSF11.

Long Name

TNF-related Activation-induced Cytokine

Alternate Names

CD254, ODF, OPGL, RANK L, RANKL, TNFSF11

Entrez Gene IDs

8600 (Human); 21943 (Mouse); 117516 (Rat); 102137946 (Cynomolgus Monkey)

Gene Symbol

TNFSF11

UniProt

AAC51762

Additional TRANCE/TNFSF11/RANK L Products

Product Documents for Human TRANCE/TNFSF11/RANK L Antibody (175707)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TRANCE/TNFSF11/RANK L Antibody (175707)

For research use only

Citations for Human TRANCE/TNFSF11/RANK L Antibody (175707)

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