Detection of Human Transferrin by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Transferrin Monoclonal Antibody (Catalog # MAB5746) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Transferrin at approximately 90 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1. It is recommended to use this antibody under non-reducing conditions only.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Transferrin, also known as serotransferrin and siderophilin, is an 80 kDa secreted glycoprotein that binds two Fe (3+) ions, transporting them from sites of absorption and heme degradation to those of storage and utilization. It is a member of the Transferrin family of proteins. Human Transferrin is synthesized as a 698 amino acid (aa) precursor that contains a 19 aa signal sequence and a 679 aa mature chain. The mature chain consists of two Transferrin-like domains, two high-affinity iron binding sites, one potential site for O-linked glycosylation, and two potential sites for N-linked glycosylation. Human Transferrin is 73% aa identical to mouse and rat Transferrin.
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