Human Transferrin Antibody Summary
Accession # AAH59367
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Transferrin by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Transferrin Monoclonal Antibody (Catalog # MAB5746) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Transferrin at approximately 90 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1. It is recommended to use this antibody under non-reducing conditions only.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Transferrin, also known as serotransferrin and siderophilin, is an 80 kDa secreted glycoprotein that binds two Fe (3+) ions, transporting them from sites of absorption and heme degradation to those of storage and utilization. It is a member of the Transferrin family of proteins. Human Transferrin is synthesized as a 698 amino acid (aa) precursor that contains a 19 aa signal sequence and a 679 aa mature chain. The mature chain consists of two Transferrin-like domains, two high-affinity iron binding sites, one potential site for O-linked glycosylation, and two potential sites for N-linked glycosylation. Human Transferrin is 73% aa identical to mouse and rat Transferrin.
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