Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TREML2/TLT-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TREML2
Gly19-Ser268
Accession # Q5T2D2

Specificity

Detects human TREML2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse TREML2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human TREML2/TLT‑2 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human TREML2/TLT‑2 Fc Chimera

Reviewed Applications

Read 4 reviews rated 4.8 using AF3259 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TREML2/TLT-2

Trem-like transcript 2 protein (TLT-2; also TREML2) is a 33 kDa (unglycosylated) type I transmembrane cell surface receptor and member of the Trem family of receptor proteins.  Human TLT-2 is synthesized as a 321 amino acid (aa) precursor that contains an18 aa signal sequence, a 250 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 32 aa cytoplasmic tail (SwissProt #: Q5T2D2). Within the ECD are an Ig-like V-type domain (aa 20-121) and one potential site of N-linked glycosylation. Mature human TLT-2 shares 49% aa sequence identity with mature mouse TLT-2. Cell surface expression of TLT-2 is seen for most of the B and myeloid cell lines (1, 2). TLT-2 is also expressed constitutively on CD8+ T cells and induced on CD4+ T cells after activation (2). TLT-2 plays a role in the innate and adaptive immune responses. In 2008, Hashiguchi et al. discovered that the B7 family member B7-H3, which also plays important roles in immune responses, specifically bound to TLT-2. According to their research, stimulation with B7-H3 transfectants preferentially up-regulated the proliferation and IFN-gamma production on CD8+ T cells (2). Also, transduction of TLT-2 into T cells resulted in enhanced IL-2 and IFN-gamma production via interactions with B7-H3, and blockade of the B7-H3:TLT-2 pathway with mAb against B7-H3 or TLT-2 efficiently inhibited contact hypersensitivity responses (2). In 2009, however, Leitner et al. extensively analyzed interaction of B7-H3 with TLT-2 and found no evidence for such an interaction (3).

References

  1. King, R.G. et al., 2006, J. Immunol. 176:6012.
  2. Hashiguchi, M. et al., 2008, Proc. Natl. Acad. Sci. USA 105:10495.
  3. Leitner, J. et al., 2009, Eur. J. Immunol. 39:1754.

Long Name

Triggering Receptor Expressed on Myeloid Cells-like 2

Alternate Names

TLT2

Entrez Gene IDs

79865 (Human); 328833 (Mouse)

Gene Symbol

TREML2

UniProt

Q5T2D2

Additional TREML2/TLT-2 Products

Product Documents for Human TREML2/TLT‑2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TREML2/TLT‑2 Antibody

For research use only

Citations for Human TREML2/TLT‑2 Antibody

Customer Reviews for Human TREML2/TLT‑2 Antibody (4)

4.8 out of 5
4 Customer Ratings
5 Stars
75%
4 Stars
25%
3 Stars
0%
2 Stars
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1 Stars
0%

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Showing  1 - 4 of 4 reviews Showing All
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  • Human TREML2/TLT-2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: 293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 03/04/2022
    1st lane: 293T cells + vector, 2nd lane: 293T cells + TLT2 expression vector exposure time: very short
    Human TREML2/TLT‑2 Antibody AF3259
  • Human TREML2/TLT-2 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: 293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 03/01/2022
    For the data, it's 293T transfected with hTREML2 plasmid, then stained with this antibody, blue: IgG isotype control, red: antibody AF3259.
    Human TREML2/TLT‑2 Antibody AF3259
  • Human TREML2/TLT‑2 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: human peripheral CD11c+ dendritic cells
    Species: Human
    Verified Customer | Posted 10/26/2015
    Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion. After primary antibody staining, cells were wash 3x and Alexa 488 labeled rabbit anti-goat IgG was added for 20 min. Cells were washed 2x and fixed in orthofix. Isotype control shaded grey, TREML2 in open histogram. <br />Specificity: Specific<br />Sensitivity: Sensitive<br />Buffer: 2% human serum, 0.5 mM EDTA in PBS<br />Dilution: 1/100
    Human TREML2/TLT‑2 Antibody AF3259
  • Human TREML2/TLT‑2 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: human peripheral CD11c+ dendritic cells
    Species: Human
    Verified Customer | Posted 10/26/2015
    Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion. After primary antibody staining, cells were wash 3x and Alexa 488 labeled rabbit anti-goat IgG was added for 20 min. Cells were washed 2x and fixed in orthofix. Isotype control shaded grey, TREML1 in open histogram. <br />Specificity: Specific<br />Sensitivity: Sensitive<br />Buffer: 2% human serum, 0.5 mM EDTA in PBS<br />Dilution: 1/100
    Human TREML2/TLT‑2 Antibody AF3259

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