Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TRIM5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TRIM5
Ala2-Gln248
Accession # Q9C035

Specificity

Detects human TRIM5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse TRIM5 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human TRIM5 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human TRIM5

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRIM5

TRIM5 belongs to the large family of TRIM proteins, which are also named RBCC proteins. These proteins contain a RING domain, one or two B-boxes (CHC3H2 zinc finger motifs), and the coiled-coil domain. Different TRIM proteins contain C-terminals that vary in length and composition. They also have different functions and cellular localization. TRIM5 possesses a B30.2 (also known as RFP-like or PRY-SPRY) C-terminal domain and exists as a homotrimer. TRIM5 is a single protein RING finger E3 ubiquitin ligase and has been shown to have antiretroviral activities. Five splice variants that have identical amino-terminal residues 1‑248, but differ in their C-terminal regions have been reported. Human TRIM5 shares 49% amino acid sequence homology with mouse TRIM5.

Long Name

Tripartite Motif-containing 5

Alternate Names

RNF88

Entrez Gene IDs

85363 (Human)

Gene Symbol

TRIM5

UniProt

Q9C035

Additional TRIM5 Products

Product Documents for Human TRIM5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human TRIM5 Antibody

For research use only

Related Research Areas

E3 Ubiquitin Ligases
Infectious Diseases

Citations for Human TRIM5 Antibody

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