Human TrkB Antibody

Catalog # Availability Size / Price Qty
MAB3971-SP
MAB3971-100
MAB3971-500
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews

Human TrkB Antibody Summary

Species Reactivity
Human
Specificity
Detects human TrkB in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) TrkA, recombinant rat TrkA, recombinant mouse (rm) TrkB, rhTrkC, or rmTrkC is observed.
Source
Monoclonal Mouse IgG1 Clone # 72509
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TrkB
Cys32-His430
Accession # Q16620
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
Human TrkB transfected C6 cells
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TrkB

The neurotrophins, including NGF, BDNF, NT-3, and NT-4/5 constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to the different members of the Trk family tyrosine kinase receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF, TrkB binds BDNF and
NT‑4/5 and TrkC binds NT-3. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Natural splice variants of the different Trks, including TrkB variants lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described. The role of the different extracellular subdomains of TrkB in mediating neurotrophin binding and discrimination is currently being investigated. At the protein sequence level, human and rat TrkB are greater than 90% identical and the proteins exhibit cross-species activity. TrkB is primarily expressed in the nervous system. However, low levels of TrkB expression have also been observed in a wide variety of tissues (pancreas, kidneys, ovary) outside the nervous system.

References
  1. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
  2. Middlemas, D.S. et al. (1991) Mol. Cell Biol. 11:143.
  3. Soppet, D. et al. (1991) Cell 65:895.
Long Name
Neurotrophic Tyrosine Kinase Receptor B
Entrez Gene IDs
4915 (Human); 18212 (Mouse); 25054 (Rat)
Alternate Names
BDNF/NT-3 growth factors receptor; EC 2.7.10; EC 2.7.10.1; GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; neurotrophic tyrosine kinase, receptor, type 2; NTRK2; TrkB tyrosine kinase; TrkB; trk-B; TRKBBDNF-tropomyosine receptor kinase B; tyrosine kinase receptor B

Product Datasheets

Citations for Human TrkB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. P75 neurotrophin receptor is sequestered in the Golgi apparatus of the U-87 MG human glioblastoma cell line.
    Authors: Giraud S, Loum E, Bessette B, Mathonnet M, Lalloue F
    Int. J. Oncol., 2011;38(2):391-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. BDNF and its receptors in human myasthenic thymus: implications for cell fate in thymic pathology.
    Authors: Berzi A, Ayata CK, Cavalcante P, Falcone C, Candiago E, Motta T, Bernasconi P, Hohlfeld R, Mantegazza R, Meinl E, Farina C
    J. Neuroimmunol., 2008;197(2):128-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Brain-derived neurotrophic factor stimulates bone/cementum-related protein gene expression in cementoblasts.
    Authors: Kajiya M, Shiba H, Fujita T, Ouhara K, Takeda K, Mizuno N, Kawaguchi H, Kitagawa M, Takata T, Tsuji K, Kurihara H
    J. Biol. Chem., 2008;283(23):16259-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human TrkB Antibody

There are currently no reviews for this product. Be the first to review Human TrkB Antibody and earn rewards!

Have you used Human TrkB Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review