Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Immunohistochemistry, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TRPS1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TRPS1
Met1-Phe222
Accession # Q9UHF7

Specificity

Detects human TRPS1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TRPS1 Antibody

Detection of Human TRPS1 antibody by Western Blot.

Detection of Human TRPS1 by Western Blot.

Western blot shows lysates of MDA-MB-453 human breast cancer cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TRPS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4838) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TRPS1 at approximately 165 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Human TRPS1 antibody by Simple WesternTM.

Detection of Human TRPS1 by Simple WesternTM.

Simple Western lane view shows lysates of MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for TRPS1 at approximately 191 kDa (as indicated) using 10 µg/mL of Goat Anti-Human TRPS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4838) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Applications for Human TRPS1 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: MCF‑7 human breast cancer cell line

Western Blot

1 µg/mL
Sample: MDA-MB-453 human breast cancer cell line and MCF-7 human breast cancer cell line

Reviewed Applications

Read 1 review rated 5 using AF4838 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRPS1

TRPS1 (also GC79) is a 160-175 kDa (predicted 140 kDa) member of the zinc finger family of transcription factors. It is ubiquitously expressed and serves to repress the expression of GATA-regulated genes. Human TRPS1 is 1281 amino acids (aa) in length. It contains nine divergent zinc-finger motifs (aa 222‑1267), the seventh of which is associated with a GATA-binding sequence (aa 896‑920) and is flanked by two NLSs (aa 886‑891 and 946‑952). Two SUMOylation sites exist (Lys1192 and Lys1201) and when utilized, they each add 40 kDa to the native MW. One alternative splice form shows premature truncation after Lys853. A second shows an alternate start site 13 aa upstream of the standard start site. Over aa 1‑222, human TRPS1 shares 95% and 89% aa identity with canine and mouse TRPS1, respectively.

Long Name

Tricho-rhino-phalangeal Syndrome Type I Protein

Alternate Names

GC79

Entrez Gene IDs

7227 (Human); 83925 (Mouse); 299897 (Rat)

Gene Symbol

TRPS1

UniProt

Q9UHF7 (Human)

Additional TRPS1 Products

Product Documents for Human TRPS1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TRPS1 Antibody

For research use only

Citations for Human TRPS1 Antibody

Customer Reviews for Human TRPS1 Antibody (1)

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  • Human TRPS1 Antibody
    Name: Anonymous
    Application: Chromatin Immunoprecipitation
    Sample Tested: MCF-7 human breast cancer cell line, ZR-75 human breast cancer cell line and T47D human breast cancer cell line
    Species: Human
    Verified Customer | Posted 06/26/2018
    https://doi.org/10.1038/s41388-018-0312-2
    Human TRPS1 Antibody AF4838

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