Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 410322
View all Trypsin 2/PRSS2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Trypsin 2/PRSS2
Ala16-Ser247
Accession # P07478

Specificity

Detects human Trypsin 2/PRSS2 in direct ELISAs and Western blots. Does not cross‑react with recombinant human (rh) Trypsin 1 or rhTrypsin 3. Recognizes both the pro and mature forms of rhTrypsin 2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Trypsin 2/PRSS2 Antibody

Detection of Human Trypsin 2/PRSS2 by Western Blot.

Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Trypsin 2/PRSS2 Monoclonal Antibody (Catalog # MAB3586) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Trypsin 2/PRSS2 at approximately 24 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.

Detection of Human Trypsin 2/PRSS2 by Western Blot.

Western blot shows recombinant human Trypsin 1, recombinant human Trypsin 2 and recombinant human Trypsin 3. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Trypsin-2/PRSS2 Monoclonal Antibody (Catalog # MAB3586) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF108). A specific band was detected for Trypsin-2/PRSS2 at approximately 24 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.

Detection of Human Trypsin 2/PRSS2 by Immunoprecipitation.

Human Trypsin 2/PRSS2 was immunoprecipitated from 500 μg of human pancreas lysates with 12.5 ug Mouse Anti-Human Trypsin 2/PRSS2 Monoclonal Antibody (Catalog # MAB3586). The Trypsin 2/PRSS2-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated human Trypsin 2/PRSS2 was detected by Western blot using 2 µg/mL of Sheep Anti-Human Trypsin Pan Specific (PRSS1/2/3) Antigen Affinity-purified Polyclonal Antibody (AF3586) under non-reducing conditions and using Western Blot Buffer Group 1.

Applications for Human Trypsin 2/PRSS2 Antibody

Application
Recommended Usage

Immunoprecipitation

12.5 µg/500 µg cell lysate
Sample: Human pancreas tissue

Western Blot

2 µg/mL
Sample: Human Pancreas Tissue and Recombinant Human Trypsin 2/PRSS2 under non-reducing conditions only

Reviewed Applications

Read 3 reviews rated 4 using MAB3586 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Trypsin 2/PRSS2

Human Trypsin 2, encoded by the PRSS2 gene, is also known as anionic Trypsin (1). Accounting for approximately 1/3 of the total trypsin content in the pancreatic juice, it is one of the trypsins produced in the pancreas. It consists of a signal peptide (residues 1‑15), a pro region (residues 16‑23), and a mature chain (residues 24‑247). Trypsin 2 is synthesized as a zymogen and secreted into the duodenal lumen, where it is activated by enteropeptidase (2). It plays a central role in digestion and activating other pro-enzymes. In human pancreatic diseases and chronic alcoholism, it is up-regulated (3).

References

  1. Emi, M. et al. (1986) Gene. 41:305.
  2. Halfon, S. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 1483-1488, Academic Press, San Diego.
  3. Rinderknecht, H. et al. (1979) Gut. 20:886.

Alternate Names

PRSS2, Tesp4, TRY8, Trypsinogen 20

Entrez Gene IDs

5645 (Human); 22072 (Mouse); 25052 (Rat)

Gene Symbol

PRSS2

UniProt

P07478

Additional Trypsin 2/PRSS2 Products

Product Documents for Human Trypsin 2/PRSS2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Trypsin 2/PRSS2 Antibody

For research use only

Citations for Human Trypsin 2/PRSS2 Antibody

Customer Reviews for Human Trypsin 2/PRSS2 Antibody (3)

4 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
0%
3 Stars
0%
2 Stars
33%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human Trypsin 2/PRSS2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Pancreas tissue
    Species: Human
    Verified Customer | Posted 12/02/2021
    Human Trypsin 2/PRSS2 Antibody MAB3586
  • Human Trypsin 2/PRSS2 Antibody
    Name: Anonymous
    Application: Luminex xMAP technology
    Sample Tested: Adult pancreas
    Species: Human
    Verified Customer | Posted 04/17/2018
    Human Trypsin 2/PRSS2 Antibody MAB3586
    Bio-Techne Response
    The customer is using this antibody in an unvalidated application. Technical Service is following up with the customer. This antibody is being used in an application we cannot guarantee.
  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: See PMID 20407433
    Species: Human
    Verified Customer | Posted 02/20/2015

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