Human Trypsin 2, encoded by the PRSS2 gene, is also known as anionic Trypsin (1). Accounting for approximately 1/3 of the total trypsin content in the pancreatic juice, it is one of the trypsins produced in the pancreas. It consists of a signal peptide (residues 1‑15), a pro region (residues 16‑23), and a mature chain (residues 24‑247). Trypsin 2 is synthesized as a zymogen and secreted into the duodenal lumen, where it is activated by enteropeptidase (2). It plays a central role in digestion and activating other pro-enzymes. In human pancreatic diseases and chronic alcoholism, it is up-regulated (3).
Human Trypsin 2/PRSS2 Antibody (410322)
R&D Systems | Catalog # MAB3586
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, Immunoprecipitation
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 410322
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Trypsin 2/PRSS2
Ala16-Ser247
Accession # P07478
Ala16-Ser247
Accession # P07478
Specificity
Detects human Trypsin 2/PRSS2 in direct ELISAs and Western blots. Does not cross‑react with recombinant human (rh) Trypsin 1 or rhTrypsin 3. Recognizes both the pro and mature forms of rhTrypsin 2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Trypsin 2/PRSS2 Antibody (410322)
Detection of Human Trypsin 2/PRSS2 by Western Blot.
Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Trypsin 2/PRSS2 Monoclonal Antibody (Catalog # MAB3586) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Trypsin 2/PRSS2 at approximately 24 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.
Detection of Human Trypsin 2/PRSS2 by Western Blot.
Western blot shows recombinant human Trypsin 1, recombinant human Trypsin 2 and recombinant human Trypsin 3. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Trypsin-2/PRSS2 Monoclonal Antibody (Catalog # MAB3586) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF108). A specific band was detected for Trypsin-2/PRSS2 at approximately 24 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.
Detection of Human Trypsin 2/PRSS2 by Immunoprecipitation.
Human Trypsin 2/PRSS2 was immunoprecipitated from 500 μg of human pancreas lysates with 12.5 ug Mouse Anti-Human Trypsin 2/PRSS2 Monoclonal Antibody (Catalog # MAB3586). The Trypsin 2/PRSS2-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated human Trypsin 2/PRSS2 was detected by Western blot using 2 µg/mL of Sheep Anti-Human Trypsin Pan Specific (PRSS1/2/3) Antigen Affinity-purified Polyclonal Antibody (AF3586) under non-reducing conditions and using Western Blot Buffer Group 1.Applications for Human Trypsin 2/PRSS2 Antibody (410322)
Application
Recommended Usage
Immunoprecipitation
12.5 µg/500 µg cell lysate
Sample: Human pancreas tissue
Sample: Human pancreas tissue
Western Blot
2 µg/mL
Sample: Human Pancreas Tissue and Recombinant Human Trypsin 2/PRSS2 under non-reducing conditions only
Sample: Human Pancreas Tissue and Recombinant Human Trypsin 2/PRSS2 under non-reducing conditions only
Reviewed Applications
Read 3 reviews rated 4 using MAB3586 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Trypsin 2/PRSS2
References
- Emi, M. et al. (1986) Gene. 41:305.
- Halfon, S. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 1483-1488, Academic Press, San Diego.
- Rinderknecht, H. et al. (1979) Gut. 20:886.
Alternate Names
PRSS2, Tesp4, TRY8, Trypsinogen 20
Gene Symbol
PRSS2
UniProt
Additional Trypsin 2/PRSS2 Products
Product Documents for Human Trypsin 2/PRSS2 Antibody (410322)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Trypsin 2/PRSS2 Antibody (410322)
For research use only
Related Research Areas
Citations for Human Trypsin 2/PRSS2 Antibody (410322)
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Customer Reviews for Human Trypsin 2/PRSS2 Antibody (410322) (3)
4 out of 5
3 Customer Ratings
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Application: Western BlotSample Tested: Pancreas tissueSpecies: HumanVerified Customer | Posted 12/02/2021
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Application: Luminex xMAP technologySample Tested: Adult pancreasSpecies: HumanVerified Customer | Posted 04/17/2018
Bio-Techne ResponseThe customer is using this antibody in an unvalidated application. Technical Service is following up with the customer. This antibody is being used in an application we cannot guarantee.
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Application: ImmunocytochemistrySample Tested: See PMID 20407433Species: HumanVerified Customer | Posted 02/20/2015
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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