Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 508417
View all Tryptophan Hydroxylase 1/TPH-1 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Tryptophan Hydroxylase 1/TPH‑1
Met102-Pro403
Accession # P17752

Specificity

Detects human Tryptophan Hydroxylase 1/TPH‑1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human Tryptophan Hydroxylase 1/TPH‑1 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Tryptophan Hydroxylase 1/TPH‑1, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Tryptophan Hydroxylase 1/TPH‑1

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Tryptophan Hydroxylase 1/TPH-1

Tryptophan Hydroxylase-1 (TPH-1) is a member of the pterin-dependent aromatic acid hydroxylase family. It catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. The amino acid sequence of human TPH-1 is 96%, 90%, and 87% identical to that of cow, mouse, and chicken.

Alternate Names

TPH-1, TPRH

Entrez Gene IDs

7166 (Human); 21990 (Mouse); 24848 (Rat)

Gene Symbol

TPH1

UniProt

P17752

Additional Tryptophan Hydroxylase 1/TPH-1 Products

Product Documents for Human Tryptophan Hydroxylase 1/TPH‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Tryptophan Hydroxylase 1/TPH‑1 Antibody

For research use only

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