Human TSP50 Antibody

Catalog # Availability Size / Price Qty
Detection of Human TSP50 by Western Blot.
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Product Details
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Human TSP50 Antibody Summary

Species Reactivity
Detects human TSP50 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse TSP50 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human TSP50
Accession # Q9UI38
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.2 µg/mL
See below
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human TSP50, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TSP50 antibody by Western Blot. View Larger

Detection of Human TSP50 by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TSP50 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2430) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TSP50 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSP50

TSP50 is a member of the peptidase S1 family of serine proteases. It is a secreted glycoprotein that may play a role in spermatogenesis and act as an oncogene.

Long Name
Testes-specific Protease 50
Entrez Gene IDs
29122 (Human); 235631 (Mouse)
Alternate Names
Cancer/testis antigen 20; CT20probable threonine protease PRSS50; EC 3.4.25.-; protease, serine, 50; Serine protease 50; testes-specific protease 50; Testis-specific protease-like protein 50; TSP50; TSP50testes specific protease 50

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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