Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2054B
View all TTF-1/NKX2-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TTF-1/NKX2-1
Ala40-Pro111
Accession # P43699

Specificity

Detects human TTF-1/NKX2-1 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human TTF-1/NKX2-1 Antibody

Detection of Human TTF-1/NKX2-1 antibody by Western Blot.

Detection of Human TTF-1/NKX2-1 by Western Blot.

Western blot shows lysates of TT human medullary thyroid cancer cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human TTF-1/NKX2-1 Monoclonal Antibody (Catalog # MAB9458) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for TTF-1/NKX2-1 at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TTF-1/NKX2-1 antibody in A549 Human Cell Line by Immunocytochemistry (ICC).

TTF-1/NKX2-1 in A549 Human Cell Line.

TTF-1/NKX2-1 was detected in immersion fixed A549 human lung carcinoma cell line using Rabbit Anti-Human TTF-1/NKX2-1 Monoclonal Antibody (Catalog # MAB9458) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human TTF-1/NKX2-1 Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line

Western Blot

1 µg/mL
Sample: TT human medullary thyroid cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TTF-1/NKX2-1

NK2 homeobox 1 (NKX2-1), also known as thyroid transcription factor 1 (TTF-1), is a 371 amino acids (aa) protein encoded by the NKX2-1 gene. Expression of NKX2-1 transcription factor is the earliest indication of the establishment of respiratory progenitors as well as thyroid epithelium in the ventral foregut endoderm. NKX2-1 is critical for the expression of many pulmonary specific genes, including surfactant proteins SP-A, -B, and –C, as well as thyroid specific genes that are important in the maintenance of the thyroid differentiation phenotype.

Long Name

NK2 Homeobox 1

Alternate Names

BCH, BHC, NKX2-1, NKX2.1, NKX2A, T/EBP, TITF1, TTF1

Entrez Gene IDs

7080 (Human); 21869 (Mouse); 25628 (Rat)

Gene Symbol

NKX2-1

UniProt

P43699

Additional TTF-1/NKX2-1 Products

Product Documents for Human TTF-1/NKX2-1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TTF-1/NKX2-1 Antibody

For research use only

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Protocols

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