Human TTF-1/NKX2-1 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
Detection of Human TTF-1/NKX2-1 by Western Blot.
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Product Details
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Human TTF-1/NKX2-1 Antibody Summary

Species Reactivity
Detects human TTF-1/NKX2-1 in direct ELISAs.
Recombinant Monoclonal Rabbit IgG Clone # 2054B
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human TTF-1/NKX2-1
Accession # P43699
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TTF-1/NKX2-1 antibody by Western Blot. View Larger

Detection of Human TTF-1/NKX2-1 by Western Blot. Western blot shows lysates of TT human medullary thyroid cancer cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human TTF-1/NKX2-1 Monoclonal Antibody (Catalog # MAB9458) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for TTF-1/NKX2-1 at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry TTF-1/NKX2-1 antibody in A549 Human Cell Line by Immunocytochemistry (ICC). View Larger

TTF-1/NKX2-1 in A549 Human Cell Line. TTF-1/NKX2-1 was detected in immersion fixed A549 human lung carcinoma cell line using Rabbit Anti-Human TTF-1/NKX2-1 Monoclonal Antibody (Catalog # MAB9458) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TTF-1/NKX2-1

NK2 homeobox 1 (NKX2-1), also known as thyroid transcription factor 1 (TTF-1), is a 371 amino acids (aa) protein encoded by the NKX2-1 gene. Expression of NKX2-1 transcription factor is the earliest indication of the establishment of respiratory progenitors as well as thyroid epithelium in the ventral foregut endoderm. NKX2-1 is critical for the expression of many pulmonary specific genes, including surfactant proteins SP-A, -B, and –C, as well as thyroid specific genes that are important in the maintenance of the thyroid differentiation phenotype.

Long Name
NK2 Homeobox 1
Entrez Gene IDs
7080 (Human); 21869 (Mouse); 25628 (Rat)
Alternate Names
BCH; benign chorea; BHC; Homeobox protein NK-2 homolog A; homeobox protein Nkx-2.1; NK2 homeobox 1; NK-2 homolog A; NKX2.1; NKX2-1; NKX2A; NKX2ANKX2.1; T/EBP; Thyroid nuclear factor 1; Thyroid transcription factor 1BHC; TITF1; TITF1TEBP; TTF1; TTF-1; TTF1BCH; TTF-1NK-2

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