Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 762102
View all Tumstatin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Tumstatin

Specificity

Detects human Tumstatin in direct ELISAs. In direct ELISAs, less than 25% cross-reactivity with recombinant human Collagen IV alpha 1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Tumstatin Antibody

Detection of Human Tumstatin antibody by Western Blot.

Detection of Human Tumstatin by Western Blot.

Western blot shows lysates of human skeletal muscle tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Tumstatin Monoclonal Antibody (Catalog # MAB7546) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tumstatin at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Tumstatin Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human skeletal muscle tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Tumstatin

Tumstatin is a naturally occurring, anti-angiogenic, anti-tumor peptide of 28 kDa that constitutes the NC1 (non-collagenous) domain of the Collagen IV a3 chain [amino acids (aa) 1427-1670 of Coll IV a3;]. It is liberated from basement membranes by MMP-9, and interacts with Integrin aVb3 on endothelial cells, causing cell cycle arrest, or apoptosis of cells that are proliferating. Human Tumstatin is also active on mouse and bovine endothelial cells. Human Tumstatin shares 91% and 89% aa sequence identity with mouse and rat Tumstatin, respectively.

Alternate Names

COL4A3

Entrez Gene IDs

1285 (Human); 12828 (Mouse)

Gene Symbol

COL4A3

Additional Tumstatin Products

Product Documents for Human Tumstatin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Tumstatin Antibody

For research use only

Citations for Human Tumstatin Antibody

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