Detection of Human Tumstatin by Western Blot.
Western blot shows lysates of human skeletal muscle tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Tumstatin Monoclonal Antibody (Catalog # MAB7546) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tumstatin at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Tumstatin is a naturally occurring, anti-angiogenic, anti-tumor peptide of 28 kDa that constitutes the NC1 (non-collagenous) domain of the Collagen IV a3 chain [amino acids (aa) 1427-1670 of Coll IV a3;]. It is liberated from basement membranes by MMP-9, and interacts with Integrin aVb3 on endothelial cells, causing cell cycle arrest, or apoptosis of cells that are proliferating. Human Tumstatin is also active on mouse and bovine endothelial cells. Human Tumstatin shares 91% and 89% aa sequence identity with mouse and rat Tumstatin, respectively.
Entrez Gene IDs:
1285 (Human); 12828 (Mouse)
COL4A3; collagen alpha-3(IV) chain; collagen IV, alpha-3 polypeptide; collagen, type IV, alpha 3 (Goodpasture antigen); Goodpasture antigen; Tumstatin
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