Human Twist-1 Antibody

Catalog # Availability Size / Price Qty
AF6230
AF6230-SP
Human Twist-1 Antibody in Data
2 Images
Product Details
Citations (1)
FAQs
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Human Twist-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Twist-1 in direct ELISAs. In direct ELISAs, less than 16% cross-reactivity with recombinant human Twist-2 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Twist-1
Met1-His202
Accession # Q15672
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry Twist-1 antibody in A549 Human Cell Line by Immunocytochemistry (ICC). View Larger

Twist‑1 in A549 Human Cell Line. Twist-1 was detected in immersion fixed A549 human lung carcinoma cell line treated with 10ng/mL of Recombinant Human TGF beta 1 (Catalog # 240-B) for 48 hours using Sheep Anti-Human Twist-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6230) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of Twist-1 antibody in HeLa Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Twist‑1 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human Twist-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6230, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL012). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Twist-1

Twist-1 (Twist-related protein 1; also H-Twist and bHLHa38) is a 25‑27 kDa class B member of the bHLH transcription factor family of proteins. It is widely expressed in embryo, and select adult cells such as white adipocytes. In fat, Twist-1 induces fatty acid oxidation via PGC-1 alpha and CPT-1, and promotes MCP-1 and TNF-alpha secretion by adipocytes. In epithelium, Twist-1 dysregulation represses E-Cadherin and induces N-Cadherin expression, resulting in an epithelial-to-mesenchymal transition that can lead to cancer. Human Twist-1 is 202 amino acids (aa) in length. It contains a bHLH domain (aa 109-164) with an embedded DNA-binding motif. Twist-1 forms homodimers, and heterodimerizes with TCF3, HAND 1 and HAND 2. Two distinct mutations exist that may impact its dimerization pattern. There is one seven aa insertion after Ile135, and a second seven aa insertion after Pro139. A third unrelated variant shows a four Gly insert after Gly92. Full-length human Twist-1 shares 98% aa identity with mouse Twist-1.

Long Name
Twist Related Protein 1 [Drosophila]
Entrez Gene IDs
7291 (Human); 22160 (Mouse); 85489 (Rat)
Alternate Names
acrocephalosyndactyly 3; ACS3; B-HLH DNA binding protein; BHLHA38; bHLHa38BPES3; BPES3; Class A basic helix-loop-helix protein 38; CRS1; H-twist; H-twistblepharophimosis, epicanthus inversus and ptosis 3; SCSBPES2; twist homolog 1 (Drosophila); TWIST homolog of drosophila; TWIST; Twist1; Twist-1; twist-related protein 1

Product Datasheets

Citation for Human Twist-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Epithelial-mesenchymal transitioned circulating tumor cells capture for detecting tumor progression.
    Authors: Satelli A, Mitra A, Brownlee Z, Xia X, Bellister S, Overman M, Kopetz S, Ellis L, Meng Q, Li S
    Clin Cancer Res, 2015;21(4):899-906.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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