Human Twist-1 Antibody Summary
Accession # Q15672
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Twist‑1 in A549 Human Cell Line. Twist-1 was detected in immersion fixed A549 human lung carcinoma cell line treated with 10ng/mL of Recombinant Human TGF beta 1 (Catalog # 240-B) for 48 hours using Sheep Anti-Human Twist-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6230) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Twist‑1 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human Twist-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6230, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL012). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Twist-1 (Twist-related protein 1; also H-Twist and bHLHa38) is a 25‑27 kDa class B member of the bHLH transcription factor family of proteins. It is widely expressed in embryo, and select adult cells such as white adipocytes. In fat, Twist-1 induces fatty acid oxidation via PGC-1 alpha and CPT-1, and promotes MCP-1 and TNF-alpha secretion by adipocytes. In epithelium, Twist-1 dysregulation represses E-Cadherin and induces N-Cadherin expression, resulting in an epithelial-to-mesenchymal transition that can lead to cancer. Human Twist-1 is 202 amino acids (aa) in length. It contains a bHLH domain (aa 109-164) with an embedded DNA-binding motif. Twist-1 forms homodimers, and heterodimerizes with TCF3, HAND 1 and HAND 2. Two distinct mutations exist that may impact its dimerization pattern. There is one seven aa insertion after Ile135, and a second seven aa insertion after Pro139. A third unrelated variant shows a four Gly insert after Gly92. Full-length human Twist-1 shares 98% aa identity with mouse Twist-1.
Citation for Human Twist-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Epithelial-mesenchymal transitioned circulating tumor cells capture for detecting tumor progression.
Authors: Satelli A, Mitra A, Brownlee Z, Xia X, Bellister S, Overman M, Kopetz S, Ellis L, Meng Q, Li S
Clin Cancer Res, 2015;21(4):899-906.
Sample Types: Whole Cells
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