Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TXNDC5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TXNDC5
Glu110-Leu432
Accession # Q8NBS9

Specificity

Detects endogenous human TXNDC5 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TXNDC5 Antibody

Detection of Human TXNDC5 antibody by Western Blot.

Detection of Human TXNDC5 by Western Blot.

Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, A549 human lung carcinoma cell line, DU145 human prostate carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human TXNDC5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4840) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TXNDC5 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human TXNDC5 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HT-29 human colon adenocarcinoma cell line, A549 human lung carcinoma cell line, DU145 human prostate carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line

Reviewed Applications

Read 1 review rated 4 using AF4840 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TXNDC5

TXNDC5 (Thioredoxin (TRX) domain-contain protein 5; also ERp46 and EndoPDI) is a 50‑55 kDa member of the disulfide isomerase family of enzymes. It is expressed in hepatocytes, and tumor plus hypoxic endothelium. TXNDC5 demonstrates thioreductase (disulfide bond reduction) activity in the ER. Mature human TXNDC5 is 400 amino acids (aa) in length and migrates anomalously in SDS-PAGE. It contains three TRX domains (aa 36‑428) and a short ER retention motif (aa 429‑432). There are three potential splice variants. One shows an alternate start site at Met109, while two others show a 16 and 45 aa substitution for the N-terminal 88 amino acids. Over aa 110‑432, human TXNDC5 is 88% aa identical to mouse TXNDC5.

Long Name

Thioredoxin Domain Containing 5

Alternate Names

EndoPDI, ERp46, TLP46

Entrez Gene IDs

81567 (Human); 105245 (Mouse)

Gene Symbol

TXNDC5

UniProt

Q8NBS9 (Human)

Additional TXNDC5 Products

Product Documents for Human TXNDC5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TXNDC5 Antibody

For research use only

Customer Reviews for Human TXNDC5 Antibody (1)

4 out of 5
1 Customer Rating
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 19925638
    Species: Human
    Verified Customer | Posted 01/08/2015

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