Human URB Antibody

Detection of URB by Western Blot Committed Cardiac Progenitors and their Signature Genes.a Schematic showing the laminin (LN) protocol that uses LN-521 + 221 as a defined extracellular matrix together with small molecule inhibitors for the differentiation of pluripotent stem cells to day 11 committed cardiac progenitors (CCPs). b Heat map of three biological replicates from bulk RNA-seq data demonstrating the canonical progenitor gene expression on days 9 and 11. Supplementary Table 1 shows the actual transcripts per million (TPM) for each gene. c Time course analysis of scRNAseq data from days 0 to 11 for known canonical gene expression: PDGFRA, GATA4, KDR, NKX2–5, ISL1, and MESP1. d Immunoblotting of canonical progenitor genes from differentiation days 7, 9, and 11. Supplementary Fig. 1 shows the quantification of each blot from five biological replicates. e Heat map of the bulk RNA-seq data using CCP signature genes at differentiation days 9 and 11. Supplementary Table 1 shows the actual TPM for each gene. f Single-cell RNA-seq data (two biological replicates) of the CCP signature gene expression in tSNE plots from days 0, 4, 7, 9, and 11. The tSNE plot at the top left shows the overall distribution of each day using different colors. g Time course analysis of scRNAseq data from days 0 to 11 for day 11 CCP gene signature markers. h Immunoblotting of CCP signature genes from differentiation days 7, 9, and 11 (5 biological replicates). Supplementary Fig. 1 shows the quantification of each blot. Comparisons between groups were performed using two-way ANOVA with Tukey post hoc analysis. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37236990), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of URB by Western Blot Committed Cardiac Progenitors and their Signature Genes.a Schematic showing the laminin (LN) protocol that uses LN-521 + 221 as a defined extracellular matrix together with small molecule inhibitors for the differentiation of pluripotent stem cells to day 11 committed cardiac progenitors (CCPs). b Heat map of three biological replicates from bulk RNA-seq data demonstrating the canonical progenitor gene expression on days 9 and 11. Supplementary Table 1 shows the actual transcripts per million (TPM) for each gene. c Time course analysis of scRNAseq data from days 0 to 11 for known canonical gene expression: PDGFRA, GATA4, KDR, NKX2–5, ISL1, and MESP1. d Immunoblotting of canonical progenitor genes from differentiation days 7, 9, and 11. Supplementary Fig. 1 shows the quantification of each blot from five biological replicates. e Heat map of the bulk RNA-seq data using CCP signature genes at differentiation days 9 and 11. Supplementary Table 1 shows the actual TPM for each gene. f Single-cell RNA-seq data (two biological replicates) of the CCP signature gene expression in tSNE plots from days 0, 4, 7, 9, and 11. The tSNE plot at the top left shows the overall distribution of each day using different colors. g Time course analysis of scRNAseq data from days 0 to 11 for day 11 CCP gene signature markers. h Immunoblotting of CCP signature genes from differentiation days 7, 9, and 11 (5 biological replicates). Supplementary Fig. 1 shows the quantification of each blot. Comparisons between groups were performed using two-way ANOVA with Tukey post hoc analysis. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37236990), licensed under a CC-BY license. Not internally tested by R&D Systems.