|Detection of Human VAP‑1/AOC3 by Western Blot. Western blot shows lysates of human lung tissue, human kidney tissue, human liver tissue, and human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VAP‑1/AOC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3957) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for VAP‑1/AOC3 at approximately 95-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
Detection of Human VAP‑1/AOC3 by Simple WesternTM. Simple Western lane view shows lysates of human lung tissue and heart tissue, loaded at 0.2 mg/mL. A specific band was detected for VAP‑1/AOC3 at approximately 123 kDa (as indicated) using 10 µg/mL of Goat Anti-Human VAP‑1/AOC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3957) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the|
12-230 kDa separation system.
Vascular Adhesion Protein-1 (VAP-1) is a copper amine oxidase with a topaquinone cofactor. VAP-1 is a Type II integral membrane protein, but a soluble form of the enzyme is present in human serum, and its level increases in diabetes and some inflammatory liver diseases (1, 2). VAP-1 catalyzes the oxidative deamination of small primary amines such as methylamine, benzylamine, and aminoacetone in a reaction that produces an aldehyde, ammonia, and H2O2 (3). The enzyme is sensitive to inhibition by semicarbazide. VAP-1 expression is highest in the endothelium of lung, heart, and intestine, but low in tissues such as brain, spleen, kidney, and liver (4). VAP-1 vascular expression is regulated at sites of inflammation through its release from intracellular granules in which the protein is stored (5). The adhesive function of VAP-1 has been demonstrated in studies showing that the protein is important for the adherence of certain lymphocyte subtypes to inflamed endothelial tissues (6). VAP-1 mediated adhesion is involved in the process of leukocyte extravasation, an important feature of inflammatory responses. The role of VAP-1 amine oxidase activity in this process is not fully defined, but it appears to be carbohydrate-dependent (7). VAP-1 is considered to be a therapeutic target for diabetes, oxidative stress, and inflammatory diseases (8).