Vasohibin is an endothelium-derived negative feedback regulator of angiogenesis. The 365 amino acid (aa), 44 kDa human Vasohibin shows 93% aa identity with mouse Vasohibin. Vasohibin has no canonical signal sequence, but the full-length form is only associated with cells, while N-terminally cleaved, active 42 kDa and 36 kDa forms are both cell-associated and secreted. Minor amounts of C-terminally cleaved 27 kDa form are inactive and lack the C-terminal heparin-binding basic domain. Vasohibin expression is upregulated by VEGF and FGF‑2, but this can be modulated by IL-1, TNF‑ alpha, or IFN‑ gamma.
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 411208
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Vasohibin isoform 1
Pro2-Val365
Accession # Q7L8A9
Pro2-Val365
Accession # Q7L8A9
Specificity
Detects human Vasohibin in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human Vasohibin Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human Vasohibin
Sample: Recombinant Human Vasohibin
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Vasohibin
Alternate Names
KIAA1036, VASH1
Gene Symbol
VASH1
UniProt
Additional Vasohibin Products
Product Documents for Human Vasohibin Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Vasohibin Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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