Human VEGFR2/KDR/Flk-1 Antibody Summary
Ala20-Glu764
Accession # AAC16450
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data

Detection of Human VEGFR2/KDR/Flk‑1 by Western Blot. Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VEGFR2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for VEGFR2/KDR/Flk-1 at approximately 200-250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

VEGFR2/KDR/Flk‑1 in Human Placenta. VEGFR2/KDR/Flk-1 was detected in immersion fixed paraffin-embedded sections of human placenta using 15 µg/mL Goat Anti-Human VEGFR2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

VEGFR2/KDR/Flk‑1 in Human Kidney. VEGFR2/KDR/Flk-1 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human VEGFR2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

VEGFR2/KDR/Flk‑1 Inhibi-tion of VEGF-dependent Cell Proliferation and Neutral-ization by Human VEGFR2/KDR/Flk‑1 Anti-body. Recombi-nant Human VEGFR2/KDR/Flk-1 Fc Chi-mera (Catalog # 357-KD) inhibits Recombinant Human VEGF165(Catalog # 293-VE) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human VEGF165(5 ng/mL) activity elicited by Recombinant Human VEGFR2/KDR/Flk-1 Fc Chi-mera (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human VEGFR2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357). The ND50 is typically 0.05-0.25 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VEGFR2/KDR/Flk-1
VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.
VEGFR2 cDNA encodes a 1356 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGFR2 is composed of a 745 aa residue extracellular domain, a 25 aa residue transmembrane domain and a 567 aa residue cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.
Product Datasheets
Citations for Human VEGFR2/KDR/Flk-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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The P-type ATPase transporter ATP7A promotes angiogenesis by limiting autophagic degradation of VEGFR2
Authors: D Ash, V Sudhahar, SW Youn, MN Okur, A Das, JP O'Bryan, M McMenamin, Y Hou, JH Kaplan, T Fukai, M Ushio-Fuka
Nature Communications, 2021;12(1):3091.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
The Role of Heparan Sulfate and Neuropilin 2 in VEGFA Signaling in Human Endothelial Tip Cells and Non-Tip Cells during Angiogenesis In Vitro
Authors: MG Dallinga, YI Habani, AWM Schimmel, GM Dallinga-T, CJF van Noorde, I Klaassen, RO Schlingema
Cells, 2021;10(4):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Elements of the Endomucin Extracellular Domain Essential for VEGF-Induced VEGFR2 Activity
Authors: Z Hu, I Cano, KL Saez-Torre, ME LeBlanc, M Saint-Geni, YS Ng, P Argüeso, PA D'Amore
Cells, 2020;9(6):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Pigment Epithelium-Derived Factor (PEDF) Receptors Are Involved in Survival of Retinal Neurons
Authors: S Bürger, J Meng, A Zwanzig, M Beck, M Pankonin, P Wiedemann, W Eichler, JD Unterlauft
International Journal of Molecular Sciences, 2020;22(1):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signaling
Authors: ST Wei, YC Huang, ML Hsieh, YJ Lin, WC Shyu, HC Chen, CH Hsieh
Cell Death Dis, 2020;11(5):307.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
ATF-2 and Tpl2 regulation of endothelial cell cycle progression and apoptosis
Authors: GW Fearnley, AM Latham, M Hollstein, AF Odell, S Ponnambala
Cell. Signal., 2019;66(0):109481.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Targeting chemoresistant colorectal cancer via systemic administration of a BMP7 variant
Authors: V Veschi, LR Mangiapane, A Nicotra, S Di Franco, E Scavo, T Apuzzo, DS Sardina, M Fiori, A Benfante, ML Colorito, G Cocorullo, F Giuliante, C Cipolla, G Pistone, MR Bongiorno, A Rizzo, CM Tate, X Wu, S Rowlinson, LF Stancato, M Todaro, R De Maria, G Stassi
Oncogene, 2019;0(0):.
Species: Xenograft
Sample Types: Whole Tissue
Applications: IHC-P -
Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis
Authors: G Genet, K Boyé, T Mathivet, R Ola, F Zhang, A Dubrac, J Li, N Genet, L Henrique G, L Benedetti, S Künzel, L Pibouin-Fr, JL Thomas, A Eichmann
Nat Commun, 2019;10(1):2350.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4
Authors: LD Volk-Drape, KL Hall, AC Wilber, S Ran
PLoS ONE, 2017;12(6):e0179257.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Endoglin prevents vascular malformation by regulating flow-induced cell migration and specification through VEGFR2 signalling
Authors: Y Jin, L Muhl, M Burmakin, Y Wang, AC Duchez, C Betsholtz, HM Arthur, L Jakobsson
Nat. Cell Biol., 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
VEGFR3 Modulates Vascular Permeability by Controlling VEGF/VEGFR2 Signaling
Authors: K Heinolaine, S Karaman, G D'Amico, T Tammela, R Sormunen, L Eklund, K Alitalo, G Zarkada
Circ. Res, 2017;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Affimer proteins are versatile and renewable affinity reagents
Authors: C Tiede, R Bedford, SJ Heseltine, G Smith, I Wijetunga, R Ross, D AlQallaf, AP Roberts, A Balls, A Curd, RE Hughes, H Martin, SR Needham, LC Zanetti-Do, Y Sadigh, TP Peacock, AA Tang, N Gibson, H Kyle, GW Platt, N Ingram, T Taylor, LP Coletta, I Manfield, M Knowles, S Bell, F Esteves, A Maqbool, RK Prasad, M Drinkhill, RS Bon, V Patel, SA Goodchild, M Martin-Fer, RJ Owens, JE Nettleship, ME Webb, M Harrison, JD Lippiat, S Ponnambala, M Peckham, A Smith, PK Ferrigno, M Johnson, MJ McPherson, DC Tomlinson
Elife, 2017;6(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
VEGF induces signalling and angiogenesis by directing VEGFR2 internalisation via macropinocytosis
J Cell Sci, 2016;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Sustained delivery of VEGF maintains innervation and promotes reperfusion in ischemic skeletal muscles via NGF/GDNF signaling.
Authors: Shvartsman, Dmitry, Storrie-White, Hannah, Lee, Kangwon, Kearney, Cathal, Brudno, Yevgeny, Ho, Nhi, Cezar, Christin, McCann, Corey, Anderson, Erin, Koullias, John, Tapia, Juan Car, Vandenburgh, Herman, Lichtman, Jeff W, Mooney, David J
Mol Ther, 2014;22(7):1243-53.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Control of angiogenesis by galectins involves the release of platelet-derived proangiogenic factors.
Authors: Etulain J, Negrotto S, Tribulatti M, Croci D, Carabelli J, Campetella O, Rabinovich G, Schattner M
PLoS ONE, 2014;9(4):e96402.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Endothelial cell adhesion to soluble vascular endothelial growth factor receptor-1 triggers a cell dynamic and angiogenic phenotype.
Authors: Orecchia A, Mettouchi A, Uva P, Simon G, Arcelli D, Avitabile S, Ragone G, Meneguzzi G, Pfenninger K, Zambruno G, Failla C
FASEB J, 2014;28(2):692-704.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Differential sensitivity of prostate tumor derived endothelial cells to sorafenib and sunitinib.
Authors: Fiorio Pla, Alessand, Brossa, Alessia, Bernardini, Michela, Genova, Tullio, Grolez, Guillaum, Villers, Arnaud, Leroy, Xavier, Prevarskaya, Natalia, Gkika, Dimitra, Bussolati, Benedett
BMC Cancer, 2014;14(0):939.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
VEGF-A isoforms differentially regulate ATF-2-dependent VCAM-1 gene expression and endothelial-leukocyte interactions.
Authors: Fearnley G, Odell A, Latham A, Mughal N, Bruns A, Burgoyne N, Homer-Vanniasinkam S, Zachary I, Hollstein M, Wheatcroft S, Ponnambalam S
Mol Biol Cell, 2014;25(16):2509-21.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
KDR identifies a conserved human and murine hepatic progenitor and instructs early liver development.
Authors: Goldman O, Han S, Sourisseau M, Dziedzic N, Hamou W, Corneo B, D'Souza S, Sato T, Kotton D, Bissig K, Kalir T, Jacobs A, Evans T, Evans M, Gouon-Evans V
Cell Stem Cell, 2013;12(6):748-60.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
MicroRNA-24 suppression of N-deacetylase/N-sulfotransferase-1 (NDST1) reduces endothelial cell responsiveness to vascular endothelial growth factor A (VEGFA).
Authors: Kasza Z, Fredlund Fuchs P, Tamm C, Eriksson A, O'Callaghan P, Heindryckx F, Spillmann D, Larsson E, Le Jan S, Eriksson I, Gerwins P, Kjellen L, Kreuger J
J Biol Chem, 2013;288(36):25956-63.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Essential role for endocytosis in the growth factor-stimulated activation of ERK1/2 in endothelial cells.
Authors: Gourlaouen, Morgane, Welti, Jonathan, Vasudev, Naveen S, Reynolds, Andrew R
J Biol Chem, 2013;288(11):7467-80.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Differential expression of Vegfr-2 and its soluble form in preeclampsia.
Authors: Munaut C, Lorquet S, Pequeux C, Coulon C, Le Goarant J, Chantraine F, Noel A, Goffin F, Tsatsaris V, Subtil D, Foidart JM
PLoS ONE, 2012;7(3):e33475.
Species: Human
Sample Types: Plasma
Applications: Western Blot -
The Down syndrome critical region gene 1 short variant promoters direct vascular bed-specific gene expression during inflammation in mice.
Authors: Minami T, Yano K, Miura M, Kobayashi M, Suehiro J, Reid PC, Hamakubo T, Ryeom S, Aird WC, Kodama T
J. Clin. Invest., 2009;119(8):2257-70.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Vascular endothelial growth factor drives autocrine epithelial cell proliferation and survival in chronic rhinosinusitis with nasal polyposis.
Authors: Lee HS, Myers A, Kim J
Am. J. Respir. Crit. Care Med., 2009;180(11):1056-67.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential.
Authors: Cianfarani F, Zambruno G, Brogelli L, Sera F, Lacal PM, Pesce M, Capogrossi MC, Failla CM, Napolitano M, Odorisio T
Am. J. Pathol., 2006;169(4):1167-82.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Enhancement of angiogenic effectors through hypoxia-inducible factor in preterm primate lung in vivo.
Authors: Asikainen TM, Waleh NS, Schneider BK, Clyman RI, White CW
Am. J. Physiol. Lung Cell Mol. Physiol., 2006;291(4):L588-95.
Species: Primate - Papio anubis (Olive Baboon)
Sample Types: Tissue Homogenates
Applications: Western Blot -
Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung.
Authors: Asikainen TM, Schneider BK, Waleh NS, Clyman RI, Ho WB, Flippin LA, Gunzler V, White CW
Proc. Natl. Acad. Sci. U.S.A., 2005;102(29):10212-7.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
VEGF164(165) as the pathological isoform: differential leukocyte and endothelial responses through VEGFR1 and VEGFR2.
Authors: Usui T, Ishida S, Yamashiro K, Kaji Y, Poulaki V, Moore J, Moore T, Amano S, Horikawa Y, Dartt D, Golding M, Shima DT, Adamis AP
Invest. Ophthalmol. Vis. Sci., 2004;45(2):368-74.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
PEDF derived from glial Muller cells: a possible regulator of retinal angiogenesis.
Authors: Eichler W, Yafai Y, Keller T, Wiedemann P, Reichenbach A
Exp. Cell Res., 2004;299(1):68-78.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis.
Authors: Giuliani N, Colla S, Lazzaretti M, Sala R, Roti G, Mancini C, Bonomini S, Lunghi P, Hojden M, Genestreti G, Svaldi M, Coser P, Fattori PP, Sammarelli G, Gazzola GC, Almici C, Caramatti C, Mangoni L, Rizzoli V
Blood, 2003;102(2):638-45.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Malignant mesothelioma growth inhibition by agents that target the VEGF and VEGF-C autocrine loops.
Authors: Masood R, Kundra A, Zhu S, Xia G, Scalia P, Smith DL, Gill PS
Int. J. Cancer, 2003;104(5):603-10.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Human herpesvirus 8 (HHV-8)-encoded cytokines induce expression of and autocrine signaling by vascular endothelial growth factor (VEGF) in HHV-8-infected primary-effusion lymphoma cell lines and mediate VEGF-independent antiapoptotic effects.
Authors: Okruzhnov Y, Nicholas J
J. Virol., 2001;75(22):10933-40.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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First lane- nontargeting, second lane- AP1 knockdown. Top ladder band 220kDa.