Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1066402
View all VHL Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human VHL
Asn150-Asp213
Accession # P40337

Specificity

Detects human VHL in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human VHL Antibody

Detection of Human VHL by Simple WesternTM.

Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cells, loaded at 0.2 mg/mL. A specific band was detected for VHL at approximately 23 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human VHL Monoclonal Antibody (Catalog # MAB11435). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

Detection of Human VHL by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cells and Daudi human Burkitt's lymphoma cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human VHL Monoclonal Antibody (Catalog # MAB11435) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for VHL at approximately 17, 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human VHL Antibody

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: K562 human chronic myelogenous leukemia cells

Western Blot

2 µg/mL
Sample: K562 human chronic myelogenous leukemia cells and Daudi human Burkitt's lymphoma cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VHL

VHL (von Hippel-Lindau disease tumor suppressor) is the substrate recognition subunit for an E3 ligase activity that ubiquitinates proteins containing hydroxyproline residues.  Targets of VHL include HIF1 alpha, beta 2 adrenergic receptor, ZHX2 and others.  In VHL-box E3 Ubiquitin ligases, VHL is bound to a heterodimer complex composed of Elongin B (ELOB) and Elongin C (ELOC) through the "BC-box motif" found in many proteins in the VHL-box and SOCS-box protein families.  ELOB/ELOC serves as an adapter between substrate recognition proteins and Cullin-2 (or Cullin-5) and RBX1 to form an active E3 ligase.  

References

  1. Kamura T., et al. (1998) Genes & Dev.  doi: 10.1101/gad.12.24.3872
  2. Okumura F., et al. (2012) Front. Oncol.  doi: 10.3389/fonc.2012.00010
  3. Xie L., et al. (2009) Sci. Signaling  doi: 10.1126/scisignal.2000444
  4. Zhang J., et al. (2018) Science  doi: 10.1126/science.aap8411

Long Name

Von Hippel-Lindau Tumor Suppressor

Alternate Names

Protein G7, pVHL

Entrez Gene IDs

7428 (Human); 22346 (Mouse)

Gene Symbol

VHL

UniProt

P40337 (Human)

Additional VHL Products

Product Documents for Human VHL Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human VHL Antibody

For research use only

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