Human VHR Antibody

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Product Details
Supplemental Products

Human VHR Antibody Summary

Species Reactivity
Detects human VHR in direct ELISAs.
Monoclonal Mouse IgG1 Clone # 237018
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human VHR
Accession # P51452
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human lung and liver

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VHR

Vaccinia Virus VH1-related Phosphatase (VHR), also known as Dual-Specificity Phosphatase 3 (DUSP3), removes inorganic phosphate groups covalently attached to tyrosine, serine and threonine residues in proteins (1). It belongs to a family of phosphatases that selectively dephosphorylate MAP kinases. VHR has been shown to dephosphorylate cellular ERK1, ERK2 and JNK, but not p38 (2, 3). Phosphorylation of VHR at tyrosine 138 by ZAP70 enhances inhibition of ERK2, suggesting a role for VHR in modulating T-cell receptor responses (3). The enzymatic mechanism for VHR has been studied in detail (4) and its X-ray crystal structure has been characterized (5). Its well-defined biochemistry has made it useful in screening assays for compounds that inhibit phosphatases (6).

  1. Ishibashi, T. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12170.
  2. Todd, J.L. et al. (1999) J. Biol. Chem. 274:13271.
  3. Alonso, A. et al. (2003) Nat. Immunol. 4:44.
  4. Zhang, Z.-Y. et al. (1995) Biochemistry 34:16088.
  5. Yuvaniyama, J. et al. (1996) Science 272:1328.
  6. Ueda, K. et al. (2002) FEBS Lett. 525:48.
Long Name
Vaccinia Virus VH1-related Phosphatase
Entrez Gene IDs
1845 (Human)
Alternate Names
dual specificity phosphatase 3; dual specificity protein phosphatase 3; Dual specificity protein phosphatase VHR; DUSP3; EC; EC; Vaccinia H1-related phosphatase; vaccinia virus phosphatase VH1-related; VHR; VHRserine/threonine specific protein phosphatase

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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