Von Willebrand factor A (vWFA) domain-related protein (WARP) is a 50 kDa glycoprotein member of the vWFA domain superfamily of extracellular matrix proteins. It is expressed in embryonic articular cartilage, skeletal muscle and basement membranes in the PNS. WARP forms disulfide-linked homodimers and multimers, and complexes with perlecan. Secreted human WARP contains a vWFA domain (aa 34‑213), two fibronectin type III domains (aa 211‑301 and 331‑421) that likely bind to the GAG modification of perlecan, and one potential site for N-linked glycosylation. There is one alternate start site at Met213. Mature human WARP shares 72% aa sequence identity with mature mouse and rat WARP.
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 559915
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human WARP
Arg19-Pro445
Accession # Q6PCB0
Arg19-Pro445
Accession # Q6PCB0
Specificity
Detects human WARP in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) WARP, rmCollagen II, or recombinant bovine Collagen I is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human WARP Antibody
Detection of Human WARP by Western Blot.
Western blot shows lysates of human prostate tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human WARP Monoclonal Antibody (Catalog # MAB6189) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for WARP at approximately 50, 40 and 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Applications for Human WARP Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Human prostate tissue
Sample: Human prostate tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: WARP
Long Name
von Willebrand factor A Domain-related Protein
Alternate Names
VWA1
Gene Symbol
VWA1
UniProt
Additional WARP Products
Product Documents for Human WARP Antibody
Product Specific Notices for Human WARP Antibody
For research use only
Related Research Areas
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Protocols
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