Human WIF-1 Antibody

Catalog # Availability Size / Price Qty
AF134
AF134-SP
Product Details
Citations (3)
FAQs
Supplemental Products
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Human WIF-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human and mouse WIF-1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human WIF-1
Gly29-Trp379
Accession # AAD25402
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human WIF‑1 (Catalog # 1341-WF)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WIF-1

WIF (Wnt Inhibitory Factor) is a secreted protein that binds to Wnt proteins and inhibits their activity. The protein is synthesized as a 379 amino acid (aa) molecule that contains an N-terminal signal sequence, 150 aa WIF domain, 5 EGF-like repeats, and a hydrophilic domain at the carboxy terminus (1). In situ hybridization analysis from the frog, Xenopus laevis, and zebrafish indicate that the message is highly expressed in presomitic mesoderm, the notochord, anterior regions of the brain, branchial arches, nasal placodes, and otic vescicles (1). WIF inhibits secondary axis induction by Wnts and promotes secondary axis induction by Chordin in Xenopus embryos (1). In vitro, WIF binds to Drosophila Wingless and Xenopus Wnt‑8 proteins (1). WIF-1 is implicated as an early event tumor suppressor in cancers of the prostate, breast, lung and bladder (2). However, WIF-1’s role in carcinogenesis may not be that simple since in other cancer types, such as colon adenocarcinoma, WIF facilitates tumorigenesis (3). Human WIF-1 shares 94% and 82% amino acid identity with mouse and frog, respectively.

References
  1. Hsieh, J-C. et al. (1999) Nature 398:431.
  2. Wissmann, C. et al. (2003) J. Pathol 201:204.
  3. Cebrat, M. et al. (2004) Cancer Lett. 206:107.
Long Name
Wnt Inhibitory Factor
Entrez Gene IDs
11197 (Human); 24117 (Mouse)
Alternate Names
WIF1; WIF-1; WNT inhibitory factor 1

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Citations for Human WIF-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Wnt Inhibitory Factor 1 Binds to and Inhibits the Activity of Sonic Hedgehog
    Authors: K Kerekes, M Trexler, L Bányai, L Patthy
    Cells, 2021-12-10;10(12):.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  2. Blockade of Wnt signaling inhibits angiogenesis and tumor growth in hepatocellular carcinoma.
    Authors: Hu J, Dong A, Fernandez-Ruiz V, Shan J, Kawa M, Martinez-Anso E, Prieto J, Qian C
    Cancer Res., 2009-08-18;69(17):6951-9.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Gene induction during differentiation of human pulmonary type II cells in vitro.
    Authors: Wade KC, Guttentag SH, Gonzales LW, Maschhoff KL, Gonzales J, Kolla V, Singhal S, Ballard PL
    Am. J. Respir. Cell Mol. Biol., 2006-02-10;34(6):727-37.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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