ZAG (zinc-alpha 2-glycoprotein; also ZA2G) is a 40 kDa, secreted member of the MHC class I family of proteins. It is produced by adipocytes and various epithelial cells that generate exocrine-type secretions. ZAG is reported to stimulate lipid breakdown and thus may play a role in lipid homeostasis. Mature human ZAG is 278 amino acids (aa) in length. It contains one MHC class I antigen region (aa 26‑201) and a C2‑type Ig‑like domain (aa 207‑292). Two alternate splice forms exist; one shows a 66 aa substitution for the C‑terminal 30 aa, and a second shows a nine Lys substitution for aa 151‑298. Mature human ZAG is 60% aa identical to mouse ZAG.
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 842025
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ZAG
Met1-Ser298
Accession # P25311
Met1-Ser298
Accession # P25311
Specificity
Detects human ZAG in ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human ZAG Antibody
Detection of Human ZAG by Western Blot.
Western blot shows human plasma and lysates of human prostate tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ZAG Monoclonal Antibody (Catalog # MAB4764) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ZAG at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human ZAG by Simple WesternTM.
Simple Western lane view shows lysates of human prostate tissue, loaded at 0.2 mg/mL. A specific band was detected for ZAG at approximately 59 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human ZAG Monoclonal Antibody (Catalog # MAB4764). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human ZAG Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: Human prostate tissue
Sample: Human prostate tissue
Western Blot
1 µg/mL
Sample: Human plasma and human prostate tissue
Sample: Human plasma and human prostate tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: ZAG
Long Name
Zinc alpha-2-Glycoprotein
Alternate Names
AZGP1, ZA2G, ZNGP1
Gene Symbol
AZGP1
UniProt
Additional ZAG Products
Product Documents for Human ZAG Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human ZAG Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
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- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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