Detects human ZNF143 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ZNF24, rhZNF206, rhZNF281, rhDC-SCRIPT/ZNF366, rhZNF423, or recombinant mouse Bcl-6 is observed.
Monoclonal Mouse IgG1 Clone # 677314
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human ZNF143 Gly459-Asp638 Accession # P52747
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
ZNF143 in BG01V Human Stem Cells. ZNF143 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human ZNF143 Monoclonal Antibody (Catalog # MAB6804) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
ZNF143 is a 69 kDa (unglycosylated) member of the GLI C2H2-type zinc-finger protein family. Human ZNF143 is 638 amino acids (aa) in length. A second isoform is produced from the deletion of residues 39-69. The protein contains seven C2H2-type zinc fingers, and is expressed in all tissues tested with greatest expression in the ovary. Functionally, ZNF143 serves as a transcriptional activator. Its other functions include acting as an activator for the gene for selenocysteine tRNA (tRNAsec), binding to the SPH motif of small nuclear RNA (snRNA) gene promoters, and participating in efficient U6 RNA polymerase III transcription through its interaction with CHD8. Human ZNF143 shares 97% aa sequence identity with mouse ZNF143.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.