Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 677314
View all ZNF143 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ZNF143
Gly459-Asp638
Accession # P52747

Specificity

Detects human ZNF143 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ZNF24, rhZNF206, rhZNF281, rhDC-SCRIPT/ZNF366, rhZNF423, or recombinant mouse Bcl-6 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human ZNF143 Antibody

ZNF143 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC).

ZNF143 in BG01V Human Stem Cells.

ZNF143 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human ZNF143 Monoclonal Antibody (Catalog # MAB6804) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human ZNF143 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells

Reviewed Applications

Read 1 review rated 5 using MAB6804 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ZNF143

ZNF143 is a 69 kDa (unglycosylated) member of the GLI C2H2-type zinc-finger protein family. Human ZNF143 is 638 amino acids (aa) in length. A second isoform is produced from the deletion of residues 39-69. The protein contains seven C2H2-type zinc fingers, and is expressed in all tissues tested with greatest expression in the ovary. Functionally, ZNF143 serves as a transcriptional activator. Its other functions include acting as an activator for the gene for selenocysteine tRNA (tRNAsec), binding to the SPH motif of small nuclear RNA (snRNA) gene promoters, and participating in efficient U6 RNA polymerase III transcription through its interaction with CHD8. Human ZNF143 shares 97% aa sequence identity with mouse ZNF143.

Long Name

Zinc Finger Protein 143

Alternate Names

pHZ-1, SBF, STAF

Entrez Gene IDs

7702 (Human); 20841 (Mouse); 361627 (Rat)

Gene Symbol

ZNF143

UniProt

P52747

Additional ZNF143 Products

Product Documents for Human ZNF143 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ZNF143 Antibody

For research use only

Customer Reviews for Human ZNF143 Antibody (1)

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  • Human ZNF143 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Neurons
    Species: Human
    Verified Customer | Posted 11/02/2021
    Human ZNF143 Antibody MAB6804

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