Human ZNF143 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human ZNF143 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ZNF24, rhZNF206, rhZNF281, rhDC-SCRIPT/ZNF366, rhZNF423, or recombinant mouse Bcl-6 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 677314
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human ZNF143
    Gly459-Asp638
    Accession # P52747
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
ZNF143 in BG01V Human Stem Cells. ZNF143 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human ZNF143 Monoclonal Antibody (Catalog # MAB6804) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ZNF143
ZNF143 is a 69 kDa (unglycosylated) member of the GLI C2H2-type zinc-finger protein family. Human ZNF143 is 638 amino acids (aa) in length. A second isoform is produced from the deletion of residues 39-69. The protein contains seven C2H2-type zinc fingers, and is expressed in all tissues tested with greatest expression in the ovary. Functionally, ZNF143 serves as a transcriptional activator. Its other functions include acting as an activator for the gene for selenocysteine tRNA (tRNAsec), binding to the SPH motif of small nuclear RNA (snRNA) gene promoters, and participating in efficient U6 RNA polymerase III transcription through its interaction with CHD8. Human ZNF143 shares 97% aa sequence identity with mouse ZNF143.
  • Long Name:
    Zinc Finger Protein 143
  • Entrez Gene IDs:
    7702 (Human); 20841 (Mouse); 361627 (Rat)
  • Alternate Names:
    hStaf; pHZ-1; SBF; SBFzinc finger protein 143 (clone pHZ-1); Selenocysteine tRNA gene transcription-activating factor; SPH-binding factor; STAF; STAFpHZ-1; transcriptional activator Staf; zinc finger protein 143; ZNF143
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