IGF-I/IGF-1 Antibody (SPM406)

Novus Biologicals | Catalog # NBP2-34361

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Rabbit

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, In vitro assay

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM406
View all IGF-I/IGF-1 Primary Antibodies »

Product Specifications

Immunogen

Purified human IGF-I/IGF-1 protein (Uniprot: P05019)

Localization

Cytoplasmic (Secreted)

Specificity

This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

7.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34409)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for IGF-I/IGF-1 Antibody (SPM406)

IGF-I/IGF-1 Antibody (SPM406)

Immunocytochemistry/ Immunofluorescence: IGF-I/IGF-1 Antibody (SPM406) [NBP2-34361] -

CD11c+ microglia expressed higher phagocytic genes, myelin supportive genes, and lipid metabolism associated genes than CD11c− microglia.A Gating strategy and the fluorescence minus one (FMO) sample to identify CD11c− and CD11c+ microglia on tMCAO 14d. qPCR for CD11c (B), Csf1 (C), Igf-1 (D), Spp1 (E), CD68 (F), and Axl (G) mRNA expression in CD11c− and CD11c+ microglia, respectively. n ≥ 3 repeats. The results of qPCR for Abca1 (H), Abcg1 (I), Apoe (J), Apoc1 (K), and Lpl (L) mRNA expression in CD11c− and CD11c+ microglia, respectively, on the 7th Day post-stroke. n = 5 repeats. M Representative images of CD11c (red), CD68 (orange), IGF-1 (gray), and MBP (green) immunostaining on the sham group and 7th Day after tMCAO. Scale bars, 100 μm. *p < 0.05, **p < 0.01, and ***p < 0.001. B–L Compared to CD11c- group. Values were mean +/- SEM. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36828819), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for IGF-I/IGF-1 Antibody (SPM406)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

0.5-1.0ug/ml
Application Notes
ELISA: For coating, purchase antibody without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT.
In-vitro Neutralization of Biological Activity of IGF-I/IGF-1: Order Ab without Azide.
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: IGF-I/IGF-1

Insulin is a pancreatic hormone that regulates glucose uptake and the synthesis of protein and fat. The insulin like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth promoting activity. IGF1 (Insulin Like Growth Factor I) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. IGF1 functions as an autocrine regulator of growth.

Long Name

Insulin-like Growth Factor I/Insulin-like Growth Factor 1

Alternate Names

IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C

Gene Symbol

IGF1

UniProt

P05019

Additional IGF-I/IGF-1 Products

Product Documents for IGF-I/IGF-1 Antibody (SPM406)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for IGF-I/IGF-1 Antibody (SPM406)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IGF-I/IGF-1 Antibody (SPM406)

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Protocols

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