IKK alpha Antibody (14A231) - Azide and BSA Free

Name: IKK alpha Antibody (14A231) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-27409
Price: 479 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-27409

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Citations (43)

Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, CyTOF-ready

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 14A231

Format

Azide and BSA Free

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Product Specifications

Immunogen

This antibody was raised against a His-tagged full-length human IKK alpha protein.

Reactivity Notes

New World Monkey

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Scientific Data Images for IKK alpha Antibody (14A231) - Azide and BSA Free

Western Blot: IKK alpha Antibody (14A231)Azide Free [NBP2-27409]

Western Blot: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409] - Total protein from various Human, Mouse and Rat cell lines were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-IKK-alpha in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image using the BSA free format of this product.

Immunocytochemistry/ Immunofluorescence: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409]

Immunocytochemistry/Immunofluorescence: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-IKK alpha Antibody (14A231) at 2 ug/mL overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409]

Immunohistochemistry-Paraffin: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409] - FFPE human kidney stained with IKKa antibody at 5 ug/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Flow Cytometry: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409]

Flow Cytometry: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409] - Intracellular staining of HEK293 cells using 0.5 ug of IKKa antibody (red) and isotype control (green). Intracellular flow kit was used for this test, and an anti-mouse IgG1 PE conjugated secondary antibody.

Western Blot: IKK alpha Antibody (14A231)Azide Free [NBP2-27409]

Western Blot: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409] - Analysis of IKK in Daudi cell lysate using IKKa monoclonal antibody at 1 ug/mL.

Immunocytochemistry/ Immunofluorescence: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409]

Immunocytochemistry/Immunofluorescence: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409] - IKK alpha antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody dilution of 1:10 was used. Image objective 40X.

Immunocytochemistry/ Immunofluorescence: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409]

Immunocytochemistry/Immunofluorescence: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-IKK alpha Antibody (14A231) at 2 ug/mL overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Simple Western: IKK alpha Antibody (14A231)Azide Free [NBP2-27409]

Simple Western: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409] - Analysis using Azide/BSA FREE version of NBP2-27409. Simple Western lane view shows a specific band for IKK alpha in 0.5 mg/mL of Daudi (left) and U937 (right) lysate. This exeriment was performed under reducing conditions using the 12-230 kDa separation system.

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Applications for IKK alpha Antibody (14A231) - Azide and BSA Free

Application

Recommended Usage

Flow (Intracellular)

reported in scientific literature (PMID 24804954)

Flow Cytometry

1ul/1 million cells

Immunohistochemistry-Frozen

reported in scientific literature (PMID 25133425)

Immunohistochemistry-Paraffin

5ug/ml

Immunoprecipitation

1-2ug/ml

Western Blot

1ug/ml

Application Notes

An 85 kDa band should be observed. In Simple Western only 10-15 uL of the recommended dilution is used per data point.

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IKK alpha

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkB is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, Ikka, Ikk2/, and Ikk3/. In vitro, Ikka and Ikk2/ can form homo- and heterodimers that can phosphorylate IkBs at the regulatory serine residues directly. Ikka and Ikk2/ are phosphorylated by NF-kB-inducing kinase (NIk) and MAP kinase kinase kinase-1 (MEkk1) (1), respectively. Targeted disruption of Ikka gene in mice results in skin and limb abnormalities and death of newborns (2,3).

Long Name

IkB Kinase alpha

Alternate Names

CHUK, IkBKalpha, IKK1, NFKBIKA, TCF16

Entrez Gene IDs

1147 (Human); 12675 (Mouse)

Gene Symbol

CHUK

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Product Specific Notices for IKK alpha Antibody (14A231) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.