IKK beta Antibody (10A9B6) [Biotin]

Name: IKK beta Antibody (10A9B6) [Biotin]
Brand: Novus Biologicals
SKU: NB100-55780
Price: 529 USD
Availability: InStock

Novus Biologicals | Catalog # NB100-55780

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Biotin

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 10A9B6

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Product Specifications

Immunogen

Full-length human IKKb/IKK2 recombinant protein was used as immunogen (NP_001547).

Reactivity Notes

Cross reacts with Human and Mouse.

Specificity

An approx. 87 kDa band should be observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Scientific Data Images for IKK beta Antibody (10A9B6) [Biotin]

Western Blot: IKK beta Antibody (10A9B6) [Biotin] [NB100-55780] - NB100-55780 was used to immunoprecipitate IKKb from 1x10^6 Daudi cells and the immuno-precipitated protein was detected by western blotting using NB100-55778. IKKb was detected as a protein of ~87 kD.

Applications for IKK beta Antibody (10A9B6) [Biotin]

Application

Recommended Usage

Western Blot

2-5 ug/ml

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IKK beta

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) (6) and MAP kinase kinase kinase-1 (MEKK1) (7), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli (9). Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo (9).

Long Name

IkB Kinase beta

Alternate Names

IkBKB, IKK2, NFKBIKB

Gene Symbol

IKBKB

Additional IKK beta Products

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Product Specific Notices for IKK beta Antibody (10A9B6) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.