IKK beta Antibody (10A9B6) - Azide and BSA Free

Name: IKK beta Antibody (10A9B6) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-33214
Price: 479 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-33214

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Gel Super Shift Assays

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 10A9B6

Format

Azide and BSA Free

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Product Specifications

Immunogen

Full-length human IKKb/IKK2 recombinant protein was used as immunogen (NP_001547).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Scientific Data Images for IKK beta Antibody (10A9B6) - Azide and BSA Free

Western Blot: IKK beta Antibody (10A9B6)Azide Free [NBP2-33214]

Western Blot: IKK beta Antibody (10A9B6) - Azide Free [NBP2-33214] - Analysis in Daudi cell lysate using IKK beta antibody at 5 ug/ml.

Flow Cytometry: IKK beta Antibody (10A9B6) - Azide Free [NBP2-33214]

Flow Cytometry: IKK beta Antibody (10A9B6) - Azide Free [NBP2-33214] - Analysis using the Alexa Fluor (R) 488 conjugate. Staining of IKK in HEK 293 cells using 0.1 ug of this antibody. Green histogram represents the isotype control, red represents the IKK antibody.

Simple Western: IKK beta Antibody (10A9B6)Azide Free [NBP2-33214]

Simple Western: IKK beta Antibody (10A9B6) - Azide Free [NBP2-33214] - Lane view shows a specific band for IKK beta in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.

Immunoprecipitation: IKK beta Antibody (10A9B6) - Azide Free [NBP2-33214]

Immunoprecipitation: IKK beta Antibody (10A9B6) - Azide Free [NBP2-33214] - Antibody was used to immunoprecipitate IKKb from 1x10^6 Daudi cells and the immuno-precipitated protein was detected by western blotting using NB100-56509. IKKb was detected as a protein of ~87 kD.

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Applications for IKK beta Antibody (10A9B6) - Azide and BSA Free

Application

Recommended Usage

Flow Cytometry

1ul/1 million cells

Gel Super Shift Assays

reported in scientific literature (PMID 19662631)

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 24825920)

Immunoprecipitation

2-5 ug/10^6 cells

Simple Western

1:12.5

Western Blot

2-5 ug/ml

Application Notes

An approx. 87 kDa band should be observed. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:12.5

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IKK beta

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli. Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo.

Long Name

IkB Kinase beta

Alternate Names

IkBKB, IKK2, NFKBIKB

Entrez Gene IDs

3551 (Human); 16150 (Mouse)

Gene Symbol

IKBKB

Additional IKK beta Products

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Product Specific Notices for IKK beta Antibody (10A9B6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.