IL-4 Antibody (BVD4-1D11) - BSA Free
Novus Biologicals | Catalog # NB100-64798
![Immunocytochemistry/ Immunofluorescence: IL-4 Antibody (BVD4-1D11) - BSA Free [NB100-64798]](https://resources.rndsystems.com/images/products/IL-4-Antibody-BVD4-1D11-Immunocytochemistry-Immunofluorescence-NB100-64798-img0003.jpg "Immunocytochemistry/ Immunofluorescence: IL-4 Antibody (BVD4-1D11) - BSA Free [NB100-64798]")
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry
Cited:
Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # BVD4-1D11
Format
BSA Free
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Product Specifications
Immunogen
Recombinant Mouse IL-4
Specificity
This is specific for mouse Interleukin-4 (IL-4). Interleukin-4 is a potent lymphoid growth factor, which stimulates the growth and survival of certain B and T cells. Clone BVD4-1D11 is reported to neutralise the effects of mouse IL-4 (3).
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for IL-4 Antibody (BVD4-1D11) - BSA Free
Immunocytochemistry/ Immunofluorescence: IL-4 Antibody (BVD4-1D11) - BSA Free [NB100-64798]
IL-4-Antibody-BVD4-1D11-Immunocytochemistry-Immunofluorescence-NB100-64798-img0003.jpg![Immunocytochemistry/ Immunofluorescence: IL-4 Antibody (BVD4-1D11) - BSA Free [NB100-64798]](https://resources.rndsystems.com/images/products/IL-4-Antibody-BVD4-1D11-Immunocytochemistry-Immunofluorescence-NB100-64798-img0004.jpg "Immunocytochemistry/ Immunofluorescence: IL-4 Antibody (BVD4-1D11) - BSA Free [NB100-64798]")
Immunocytochemistry/ Immunofluorescence: IL-4 Antibody (BVD4-1D11) - BSA Free [NB100-64798]
IL-4-Antibody-BVD4-1D11-Immunocytochemistry-Immunofluorescence-NB100-64798-img0004.jpg![Western Blot: IL-4 Antibody (BVD4-1D11)BSA Free [NB100-64798]](https://resources.rndsystems.com/images/products/IL-4-Antibody-BVD4-1D11-Western-Blot-NB100-64798-img0002.jpg "Western Blot: IL-4 Antibody (BVD4-1D11)BSA Free [NB100-64798]")
Western Blot: IL-4 Antibody (BVD4-1D11)BSA Free [NB100-64798]
Western Blot: IL-4 Antibody (BVD4-1D11) [NB100-64798] - Analysis of mouse IL-4 recombinant protein probed with RAT ANTI MOUSE INTERLEUKIN-4 followed by F(ab')2 RABBIT ANTI RAT IgG:HRP.Applications for IL-4 Antibody (BVD4-1D11) - BSA Free
Application
Recommended Usage
ELISA
1 ug/ml-5 ug/ml
Flow Cytometry
1:10-1:50
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Western Blot
1 ug/ml-5 ug/ml
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IL-4
Long Name
Interleukin 4
Alternate Names
BCDF, BCGF-1, BCGF1, BSF-1, BSF1, IL4, IL4E12
Gene Symbol
IL4
UniProt
Additional IL-4 Products
Product Documents for IL-4 Antibody (BVD4-1D11) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IL-4 Antibody (BVD4-1D11) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for IL-4 Antibody (BVD4-1D11) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
IL-4 Signaling Pathways
IL-4 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
IL-9 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
IL-21 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
