Key Product Details

Species Reactivity

Rat

Applications

ELISA Standard (Matched Pair), ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair)

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 53325
View all IL-6 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat IL‑6
Phe25-Thr211
Accession # P20607

Specificity

Detects rat IL-6 in ELISAs. In sandwich ELISAs, shows less than 1% cross-reactivity with recombinant mouse IL-6, recombinant human IL‑6, and recombinant porcine IL-6.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for IL-6 Antibody (53325) [PE]

Application
Recommended Usage

ELISA Capture (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Detection (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Standard (Matched Pair)

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IL-6

IL-6 is a multifunctional cytokine that has an important role in host defense by regulating immune and inflammatory responses. It is expressed by a variety of normal and transformed cells including T cells, B cells, monocytes/macrophages, fibroblasts and various tumor cells. IL-6 has diverse biological functions, it stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. Recombinant human IL-6 is a 20.9 kDa protein containing 184 amino acid residues. Human IL-6 is active on murine cells but murine IL-6 is inactive on human cells.

Long Name

Interleukin 6

Alternate Names

BSF-2, BSF2, IFNB2, IL6, MGI-2A

Gene Symbol

IL6

Additional IL-6 Products

Product Documents for IL-6 Antibody (53325) [PE]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for IL-6 Antibody (53325) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for IL-6 Antibody (53325) [PE]

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    • Q: I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
      A: We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.
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