Rat IL-6 Antibody (53325)

R&D Systems | Catalog # MAB506

R&D Systems

Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

ELISA Capture (Matched Antibody Pair)

Cited:

ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 53325
View all IL-6 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat IL‑6
Phe25-Thr211
Accession # P20607

Specificity

Detects rat IL-6 in ELISAs. In sandwich ELISAs, shows less than 1% cross-reactivity with recombinant mouse IL-6, recombinant human IL‑6, and recombinant porcine IL-6.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Rat IL-6 Antibody (53325)

Rat IL-6 ELISA Standard Curve

Rat IL-6 ELISA Standard Curve

Recombinant Rat IL‑6 (Catalog # 506-RL) was serially diluted and captured by Mouse Anti-Rat IL‑6 Monoclonal Antibody (Catalog # MAB506) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Rat IL-6 Antibody (53325)

Rat IL-6 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Rat IL‑6 Biotinylated Antibody (Catalog # BAF506)
  • Standard: Recombinant Rat IL-6 Protein (Catalog # 506-RL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using MAB506 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-6

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature rat IL-6 is 187 amino acids (aa) in length that is typically expressed as a 22-28 kDA molecular weight protein. Rat IL-6 shares 39% and 85% aa sequence identity with human and mouse IL-6, respectively. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, function to drive the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

Long Name

Interleukin 6

Alternate Names

BSF-2, BSF2, IFNB2, IL6, MGI-2A

Entrez Gene IDs

3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)

Gene Symbol

IL6

UniProt

P20607

Additional IL-6 Products

Product Documents for Rat IL-6 Antibody (53325)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat IL-6 Antibody (53325)

For research use only

Citations for Rat IL-6 Antibody (53325)

Customer Reviews for Rat IL-6 Antibody (53325) (1)

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  • Rat IL-6 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Rat
    Verified Customer | Posted 11/08/2017
    This antibody was used as a capture in an ELISA to measure rat IL-6. The BAF506 antibody was used as the detection.
    Rat IL-6 Antibody (53325) MAB506

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