Key Product Details

Species Reactivity

Validated:

Influenza A Virus H1N1

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry, Simple Western

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2908F
View all Hemagglutinin Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell, HEK293-derived influenza a virus H1N1 Hemagglutinin with a C-terminal 6-His tag
Met1-Gln529
Accession # YP_009118626

Specificity

Detects Influenza A Virus H1N1 Hemagglutinin in direct ELISA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Influenza A Virus H1N1 Hemagglutinin Antibody

Detection of Influenza A Virus H1N1 Hemagglutinin by Western Blot.

Western blot shows lysates of rvInflluenza A H1N1 Hemagglutinin. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Influenza A Virus H1N1 Hemagglutinin Monoclonal Antibody (Catalog # MAB113961) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Hemagglutinin at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1

Detection of Hemagglutinin in CHO transfected cells (positive) and Wild type CHO (negative) cells.

Hemagglutinin was detected in immersion fixed CHO Chinese hamster ovary transfected cells (positive) and absent in Wild type CHO Chinese hamster ovary cells (negative) using Rabbit Anti-Influenza A Virus H1N1 Hemagglutinin Monoclonal Antibody (Catalog # MAB113961) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Influenza A Virus H1N1 Hemagglutinin by Simple WesternTM.

Simple Western lane view shows lysates of rvInfluenza A H1N1 Hemagluttinin, loaded at 200 ng/ml. A specific band was detected for Hemagglutinin at approximately 111 kDa (as indicated) using 25 µg/mL of Rabbit Anti-Influenza A Virus H1N1 Hemagglutinin Monoclonal Antibody (Catalog # MAB113961). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Influenza A Virus H1N1 Hemagglutinin Antibody

Application
Recommended Usage

Immunocytochemistry

3-15 µg/mL
Sample: Immersion fixed CHO transfected cells (positive) and absent in Wild type CHO (negative) cells

Simple Western

25 µg/mL
Sample:
rvInfluenza A H1N1 Hemagluttinin

Western Blot

1 µg/mL
Sample: rvInflluenza A H1N1 Hemagglutinin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Hemagglutinin

Influenza hemagglutinin (HA) is a homotrimeric glycoprotein found on the surface of influenza viruses. HA is responsible for binding influenza virus to sialic acid on the surface of target cells. HA is also responsible for the fusion of the viral envelope with the late endosomal protein when it is exposed to low pH. H1N1 is a subtype of Hemagglutinin in influenza A which also includes Neurominidase protein on its surface. 

References

  1. Russell, R.J. Kerry, P.S. Stevens, D.J. Steinhauer, D.A. Martin, S.R. Gamblin, S.H. Skehel, J.J. (November, 2008). "Structure of Invluenza Hemagllutinin in Complex with an Inhibitor of Membrane Fusion". Proceedings of the National Academy of Sciences of the United States of America. 105(46):17736-41.
  2. Edinger, T.O. Pohl, O. Sertz, S. (February, 2014). "Entry of Influenza A. Virus: Host Factors and Antiviral Targets" (PDF). The Journal of General Virology. 95(Pt 2):263-277.
  3. Horvath, P. Helenius, A. Yamauchi, Y. Banerjee, I. 12, July 2013. "High-Content Analysis of Sequential Events during the Early Phase of Influenza A Virus Infection". PLOS ONE. 8(7):e68450.
  4. "Influenza Type A Viruses". Avian Influenza (Flu). CDC. 19, April 2017. Retrieved 27, August, 2018.

Long Name

Hemagglutinin

Alternate Names

HA

Entrez Gene IDs

23308115 (Influenza A Virus H1N1)

Gene Symbol

HA

UniProt

YP_009118626

Additional Hemagglutinin Products

Product Documents for Influenza A Virus H1N1 Hemagglutinin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Influenza A Virus H1N1 Hemagglutinin Antibody

For research use only

Related Research Areas

Infectious Diseases

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Protocols

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