MEK1 [p Ser298] Antibody (SR1774) - BSA Free

Name: MEK1 [p Ser298] Antibody (SR1774) - BSA Free
Brand: Novus Biologicals
SKU: NBP3-21558
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-21558

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1774 expressed in HEK293

Format

BSA Free

View all MEK1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human phospho-MEK1 [Ser298] (Uniprot #: Q02750)

Modification

p Ser 298

Specificity

Detects endogenous levels of phospho-MEK1 [Ser298]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MEK1 [p Ser298] Antibody (SR1774) - BSA Free

Western Blot: MEK1 [p Ser 298] Antibody (SR1774) [NBP3-21558] -

Western Blot: MEK1 [p Ser 298] Antibody (SR1774) [NBP3-21558] - Western blot analysis using NBP3-21558 in (1) HeLa cell treated with LP lysate; (2) HeLa cell lysate.

Applications for MEK1 [p Ser298] Antibody (SR1774) - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MEK1

MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992) Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).

Long Name

Mitogen-activated Protein Kinase Kinase 1

Alternate Names

MAP2K1, MKK1

Gene Symbol

MAP2K1

Additional MEK1 Products

Product Documents for MEK1 [p Ser298] Antibody (SR1774) - BSA Free

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Certificate of Analysis

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Product Specific Notices for MEK1 [p Ser298] Antibody (SR1774) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.