Activins belong to the TGF-beta superfamily. They are homo- or heterodimers of activin beta subunits. At least four mammalian beta subunits, A, B, C, and E, have been described. The beta c subunit homodimerizes to form Activin CC. A naturally occuring Activin AC heterodimer has also been reported (1).
Mouse Activin C beta C subunit Antibody
R&D Systems | Catalog # AF489
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Transgenic Mouse
Applications
Validated:
Western Blot
Cited:
In vivo assay
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant mouse Activin C peptide
aa 236-352
aa 236-352
Specificity
Detects mouse Activin C beta C subunit in direct ELISAs and Western blots. In direct ELISA, approximately 5% cross-reactivity with recombinant human (rh) Activin A and rhInhibin A is observed and no cross-reactivity with rhInhibin B is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse Activin C beta C subunit Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Activin C beta C subunit
Sample: Recombinant Mouse Activin C beta C subunit
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Activin C
References
- Mellor, S. et al. (2003) Endocrinology 144:4410.
Alternate Names
INHBC
Gene Symbol
INHBC
Additional Activin C Products
Product Documents for Mouse Activin C beta C subunit Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Activin C beta C subunit Antibody
For research use only
Related Research Areas
Citations for Mouse Activin C beta C subunit Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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